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Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities
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| Zeitschriftentitel: | The EuroBiotech Journal |
|---|---|
| Personen und Körperschaften: | , , , , , , , |
| In: | The EuroBiotech Journal, 3, 2019, 1, S. 10-18 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Walter de Gruyter GmbH
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| Schlagwörter: |
| author_facet |
Eze, Peter M. Nnanna, Joy C. Okezie, Ugochukwu Buzugbe, Happiness S. Abba, Chika C. Chukwunwejim, Chidimma R. Okoye, Festus B. C. Esimone, Charles O. Eze, Peter M. Nnanna, Joy C. Okezie, Ugochukwu Buzugbe, Happiness S. Abba, Chika C. Chukwunwejim, Chidimma R. Okoye, Festus B. C. Esimone, Charles O. |
|---|---|
| author |
Eze, Peter M. Nnanna, Joy C. Okezie, Ugochukwu Buzugbe, Happiness S. Abba, Chika C. Chukwunwejim, Chidimma R. Okoye, Festus B. C. Esimone, Charles O. |
| spellingShingle |
Eze, Peter M. Nnanna, Joy C. Okezie, Ugochukwu Buzugbe, Happiness S. Abba, Chika C. Chukwunwejim, Chidimma R. Okoye, Festus B. C. Esimone, Charles O. The EuroBiotech Journal Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities Genetics Molecular Biology Biomedical Engineering Molecular Medicine Food Science Biotechnology |
| author_sort |
eze, peter m. |
| spelling |
Eze, Peter M. Nnanna, Joy C. Okezie, Ugochukwu Buzugbe, Happiness S. Abba, Chika C. Chukwunwejim, Chidimma R. Okoye, Festus B. C. Esimone, Charles O. 2564-615X Walter de Gruyter GmbH Genetics Molecular Biology Biomedical Engineering Molecular Medicine Food Science Biotechnology http://dx.doi.org/10.2478/ebtj-2019-0002 <jats:title>Abstract</jats:title> <jats:p>Endophytic fungi associated with Nigerian plants have recently generated significant interest in drug discovery programmes due to their immense potential to contribute to the discovery of new bioactive compounds. This study was carried out to investigate the secondary metabolites of endophytic fungi isolated from leaves of <jats:italic>Newbouldia laevis, Ocimum gratissimum</jats:italic>, and <jats:italic>Carica papaya</jats:italic> The plants were collected from Agulu, Anambra State, South-East Nigeria. Endophytic fungal isolation, fungal fermentation; and extraction of secondary metabolites were carried out using standard methods. The crude extracts were screened for antimicrobial activities using the agar well diffusion method, and were also subjected to high performance liquid chromatography (HPLC) analysis to identify their constituents. A total of five endophytic fungi was isolated, two from <jats:italic>N. laevis</jats:italic> (NL-L1 and NL-L2), one from <jats:italic>O. gratissimum</jats:italic> (SL-L1), and two from <jats:italic>C. papaya</jats:italic> (PPL-LAC and PPL-LE2). In the antimicrobial assay, the extracts of NL-L2, SL-L1, and PPL-LE2 displayed mild antibacterial activity against both Gram negative and Gram positive test bacteria. PPL-LAC extract showed mild activity only against <jats:italic>S. aureus</jats:italic>, while no antimicrobial activity was recorded for NL-L1 extract. All the endophytic fungal extracts showed no activity against the test fungi <jats:italic>C. albicans</jats:italic> and <jats:italic>A. fumigatus</jats:italic> HPLC analysis of the fungal extracts revealed the presence of ethyl 4-hydroxyphenyl acetate and ferulic acid in NL-L1; ruspolinone in NL-L2; protocatechuic acid, scytalone, and cladosporin in SL-L1; indole-3-acetic acid and indole-3-carbaldehyde in PPL-LE2; and indole-3-acetic acid in PPL-LAC. The findings of this study revealed the potentials possessed by these plants as source of endophytes that express biological active compounds. These endophytes hold key of possibilities to the discovery of novel molecules for pharmaceutical, agricultural and industrial applications.</jats:p> Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities The EuroBiotech Journal |
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Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities |
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Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities |
| title_full |
Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities |
| title_fullStr |
Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities |
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Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities |
| title_short |
Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities |
| title_sort |
screening of metabolites from endophytic fungi of some nigerian medicinal plants for antimicrobial activities |
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Genetics Molecular Biology Biomedical Engineering Molecular Medicine Food Science Biotechnology |
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http://dx.doi.org/10.2478/ebtj-2019-0002 |
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2019 |
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10-18 |
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<jats:title>Abstract</jats:title>
<jats:p>Endophytic fungi associated with Nigerian plants have recently generated significant interest in drug discovery programmes due to their immense potential to contribute to the discovery of new bioactive compounds. This study was carried out to investigate the secondary metabolites of endophytic fungi isolated from leaves of <jats:italic>Newbouldia laevis, Ocimum gratissimum</jats:italic>, and <jats:italic>Carica papaya</jats:italic> The plants were collected from Agulu, Anambra State, South-East Nigeria. Endophytic fungal isolation, fungal fermentation; and extraction of secondary metabolites were carried out using standard methods. The crude extracts were screened for antimicrobial activities using the agar well diffusion method, and were also subjected to high performance liquid chromatography (HPLC) analysis to identify their constituents. A total of five endophytic fungi was isolated, two from <jats:italic>N. laevis</jats:italic> (NL-L1 and NL-L2), one from <jats:italic>O. gratissimum</jats:italic> (SL-L1), and two from <jats:italic>C. papaya</jats:italic> (PPL-LAC and PPL-LE2). In the antimicrobial assay, the extracts of NL-L2, SL-L1, and PPL-LE2 displayed mild antibacterial activity against both Gram negative and Gram positive test bacteria. PPL-LAC extract showed mild activity only against <jats:italic>S. aureus</jats:italic>, while no antimicrobial activity was recorded for NL-L1 extract. All the endophytic fungal extracts showed no activity against the test fungi <jats:italic>C. albicans</jats:italic> and <jats:italic>A. fumigatus</jats:italic> HPLC analysis of the fungal extracts revealed the presence of ethyl 4-hydroxyphenyl acetate and ferulic acid in NL-L1; ruspolinone in NL-L2; protocatechuic acid, scytalone, and cladosporin in SL-L1; indole-3-acetic acid and indole-3-carbaldehyde in PPL-LE2; and indole-3-acetic acid in PPL-LAC. The findings of this study revealed the potentials possessed by these plants as source of endophytes that express biological active compounds. These endophytes hold key of possibilities to the discovery of novel molecules for pharmaceutical, agricultural and industrial applications.</jats:p> |
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| author | Eze, Peter M., Nnanna, Joy C., Okezie, Ugochukwu, Buzugbe, Happiness S., Abba, Chika C., Chukwunwejim, Chidimma R., Okoye, Festus B. C., Esimone, Charles O. |
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| description | <jats:title>Abstract</jats:title> <jats:p>Endophytic fungi associated with Nigerian plants have recently generated significant interest in drug discovery programmes due to their immense potential to contribute to the discovery of new bioactive compounds. This study was carried out to investigate the secondary metabolites of endophytic fungi isolated from leaves of <jats:italic>Newbouldia laevis, Ocimum gratissimum</jats:italic>, and <jats:italic>Carica papaya</jats:italic> The plants were collected from Agulu, Anambra State, South-East Nigeria. Endophytic fungal isolation, fungal fermentation; and extraction of secondary metabolites were carried out using standard methods. The crude extracts were screened for antimicrobial activities using the agar well diffusion method, and were also subjected to high performance liquid chromatography (HPLC) analysis to identify their constituents. A total of five endophytic fungi was isolated, two from <jats:italic>N. laevis</jats:italic> (NL-L1 and NL-L2), one from <jats:italic>O. gratissimum</jats:italic> (SL-L1), and two from <jats:italic>C. papaya</jats:italic> (PPL-LAC and PPL-LE2). In the antimicrobial assay, the extracts of NL-L2, SL-L1, and PPL-LE2 displayed mild antibacterial activity against both Gram negative and Gram positive test bacteria. PPL-LAC extract showed mild activity only against <jats:italic>S. aureus</jats:italic>, while no antimicrobial activity was recorded for NL-L1 extract. All the endophytic fungal extracts showed no activity against the test fungi <jats:italic>C. albicans</jats:italic> and <jats:italic>A. fumigatus</jats:italic> HPLC analysis of the fungal extracts revealed the presence of ethyl 4-hydroxyphenyl acetate and ferulic acid in NL-L1; ruspolinone in NL-L2; protocatechuic acid, scytalone, and cladosporin in SL-L1; indole-3-acetic acid and indole-3-carbaldehyde in PPL-LE2; and indole-3-acetic acid in PPL-LAC. The findings of this study revealed the potentials possessed by these plants as source of endophytes that express biological active compounds. These endophytes hold key of possibilities to the discovery of novel molecules for pharmaceutical, agricultural and industrial applications.</jats:p> |
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| spelling | Eze, Peter M. Nnanna, Joy C. Okezie, Ugochukwu Buzugbe, Happiness S. Abba, Chika C. Chukwunwejim, Chidimma R. Okoye, Festus B. C. Esimone, Charles O. 2564-615X Walter de Gruyter GmbH Genetics Molecular Biology Biomedical Engineering Molecular Medicine Food Science Biotechnology http://dx.doi.org/10.2478/ebtj-2019-0002 <jats:title>Abstract</jats:title> <jats:p>Endophytic fungi associated with Nigerian plants have recently generated significant interest in drug discovery programmes due to their immense potential to contribute to the discovery of new bioactive compounds. This study was carried out to investigate the secondary metabolites of endophytic fungi isolated from leaves of <jats:italic>Newbouldia laevis, Ocimum gratissimum</jats:italic>, and <jats:italic>Carica papaya</jats:italic> The plants were collected from Agulu, Anambra State, South-East Nigeria. Endophytic fungal isolation, fungal fermentation; and extraction of secondary metabolites were carried out using standard methods. The crude extracts were screened for antimicrobial activities using the agar well diffusion method, and were also subjected to high performance liquid chromatography (HPLC) analysis to identify their constituents. A total of five endophytic fungi was isolated, two from <jats:italic>N. laevis</jats:italic> (NL-L1 and NL-L2), one from <jats:italic>O. gratissimum</jats:italic> (SL-L1), and two from <jats:italic>C. papaya</jats:italic> (PPL-LAC and PPL-LE2). In the antimicrobial assay, the extracts of NL-L2, SL-L1, and PPL-LE2 displayed mild antibacterial activity against both Gram negative and Gram positive test bacteria. PPL-LAC extract showed mild activity only against <jats:italic>S. aureus</jats:italic>, while no antimicrobial activity was recorded for NL-L1 extract. All the endophytic fungal extracts showed no activity against the test fungi <jats:italic>C. albicans</jats:italic> and <jats:italic>A. fumigatus</jats:italic> HPLC analysis of the fungal extracts revealed the presence of ethyl 4-hydroxyphenyl acetate and ferulic acid in NL-L1; ruspolinone in NL-L2; protocatechuic acid, scytalone, and cladosporin in SL-L1; indole-3-acetic acid and indole-3-carbaldehyde in PPL-LE2; and indole-3-acetic acid in PPL-LAC. The findings of this study revealed the potentials possessed by these plants as source of endophytes that express biological active compounds. These endophytes hold key of possibilities to the discovery of novel molecules for pharmaceutical, agricultural and industrial applications.</jats:p> Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities The EuroBiotech Journal |
| spellingShingle | Eze, Peter M., Nnanna, Joy C., Okezie, Ugochukwu, Buzugbe, Happiness S., Abba, Chika C., Chukwunwejim, Chidimma R., Okoye, Festus B. C., Esimone, Charles O., The EuroBiotech Journal, Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities, Genetics, Molecular Biology, Biomedical Engineering, Molecular Medicine, Food Science, Biotechnology |
| title | Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities |
| title_full | Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities |
| title_fullStr | Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities |
| title_full_unstemmed | Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities |
| title_short | Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities |
| title_sort | screening of metabolites from endophytic fungi of some nigerian medicinal plants for antimicrobial activities |
| title_unstemmed | Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities |
| topic | Genetics, Molecular Biology, Biomedical Engineering, Molecular Medicine, Food Science, Biotechnology |
| url | http://dx.doi.org/10.2478/ebtj-2019-0002 |