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Zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (MTF-1)
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| Zeitschriftentitel: | Biochemistry and Cell Biology |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | Biochemistry and Cell Biology, 83, 2005, 2, S. 221-229 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Canadian Science Publishing
|
| Schlagwörter: |
| author_facet |
Kindermann, Birgit Döring, Frank Budczies, Jan Daniel, Hannelore Kindermann, Birgit Döring, Frank Budczies, Jan Daniel, Hannelore |
|---|---|
| author |
Kindermann, Birgit Döring, Frank Budczies, Jan Daniel, Hannelore |
| spellingShingle |
Kindermann, Birgit Döring, Frank Budczies, Jan Daniel, Hannelore Biochemistry and Cell Biology Zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (MTF-1) Cell Biology Molecular Biology Biochemistry |
| author_sort |
kindermann, birgit |
| spelling |
Kindermann, Birgit Döring, Frank Budczies, Jan Daniel, Hannelore 0829-8211 1208-6002 Canadian Science Publishing Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1139/o04-133 <jats:p>Zinc is an essential trace element that serves as a structural constituent of a large number of transcription factors, which explains its pivotal role in the control of gene expression. Previous studies investigating the effect of zinc deficiency and zinc supplementation on gene expression in the human adenocarcinoma cell line HT-29 led to the identification of a considerable number of genes responding to alterations in cellular zinc status with changes in steady state mRNA levels. For 9 of 20 genes from these previous screenings that were studied in more detail, mRNA steady state levels responded to both high and low media zinc concentrations. As they are primarily zinc-dependent, we assessed whether these genes are controlled by the zinc-finger metal transcription factor MTF-1. To test this hypothesis we generated a doxycyline-inducible Tet-On HT-29 cell line overexpressing MTF-1. Using this conditional expression system, we present evidence that Kruppel-like factor 4 (klf4), hepatitis A virus cellular receptor 1 (hhav), and complement factor B (cfbp) are 3 potential new target genes of MTF-1. To support this, we used in silico analysis to screen for metal-responsive elements (MREs) within promotors of zinc-sensitive genes. We conclude that zinc responsiveness of klf4, hhav, and cfbp in HT-29 cells is mediated at least in part by MTF-1.Key words: zinc-sensitive genes, target genes, MTF-1, HT-29 cells, metal-response element.</jats:p> Zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (MTF-1) Biochemistry and Cell Biology |
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| title |
Zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (MTF-1) |
| title_unstemmed |
Zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (MTF-1) |
| title_full |
Zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (MTF-1) |
| title_fullStr |
Zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (MTF-1) |
| title_full_unstemmed |
Zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (MTF-1) |
| title_short |
Zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (MTF-1) |
| title_sort |
zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (mtf-1) |
| topic |
Cell Biology Molecular Biology Biochemistry |
| url |
http://dx.doi.org/10.1139/o04-133 |
| publishDate |
2005 |
| physical |
221-229 |
| description |
<jats:p>Zinc is an essential trace element that serves as a structural constituent of a large number of transcription factors, which explains its pivotal role in the control of gene expression. Previous studies investigating the effect of zinc deficiency and zinc supplementation on gene expression in the human adenocarcinoma cell line HT-29 led to the identification of a considerable number of genes responding to alterations in cellular zinc status with changes in steady state mRNA levels. For 9 of 20 genes from these previous screenings that were studied in more detail, mRNA steady state levels responded to both high and low media zinc concentrations. As they are primarily zinc-dependent, we assessed whether these genes are controlled by the zinc-finger metal transcription factor MTF-1. To test this hypothesis we generated a doxycyline-inducible Tet-On HT-29 cell line overexpressing MTF-1. Using this conditional expression system, we present evidence that Kruppel-like factor 4 (klf4), hepatitis A virus cellular receptor 1 (hhav), and complement factor B (cfbp) are 3 potential new target genes of MTF-1. To support this, we used in silico analysis to screen for metal-responsive elements (MREs) within promotors of zinc-sensitive genes. We conclude that zinc responsiveness of klf4, hhav, and cfbp in HT-29 cells is mediated at least in part by MTF-1.Key words: zinc-sensitive genes, target genes, MTF-1, HT-29 cells, metal-response element.</jats:p> |
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| author | Kindermann, Birgit, Döring, Frank, Budczies, Jan, Daniel, Hannelore |
| author_facet | Kindermann, Birgit, Döring, Frank, Budczies, Jan, Daniel, Hannelore, Kindermann, Birgit, Döring, Frank, Budczies, Jan, Daniel, Hannelore |
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| description | <jats:p>Zinc is an essential trace element that serves as a structural constituent of a large number of transcription factors, which explains its pivotal role in the control of gene expression. Previous studies investigating the effect of zinc deficiency and zinc supplementation on gene expression in the human adenocarcinoma cell line HT-29 led to the identification of a considerable number of genes responding to alterations in cellular zinc status with changes in steady state mRNA levels. For 9 of 20 genes from these previous screenings that were studied in more detail, mRNA steady state levels responded to both high and low media zinc concentrations. As they are primarily zinc-dependent, we assessed whether these genes are controlled by the zinc-finger metal transcription factor MTF-1. To test this hypothesis we generated a doxycyline-inducible Tet-On HT-29 cell line overexpressing MTF-1. Using this conditional expression system, we present evidence that Kruppel-like factor 4 (klf4), hepatitis A virus cellular receptor 1 (hhav), and complement factor B (cfbp) are 3 potential new target genes of MTF-1. To support this, we used in silico analysis to screen for metal-responsive elements (MREs) within promotors of zinc-sensitive genes. We conclude that zinc responsiveness of klf4, hhav, and cfbp in HT-29 cells is mediated at least in part by MTF-1.Key words: zinc-sensitive genes, target genes, MTF-1, HT-29 cells, metal-response element.</jats:p> |
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| imprint_str_mv | Canadian Science Publishing, 2005 |
| institution | DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14 |
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| spelling | Kindermann, Birgit Döring, Frank Budczies, Jan Daniel, Hannelore 0829-8211 1208-6002 Canadian Science Publishing Cell Biology Molecular Biology Biochemistry http://dx.doi.org/10.1139/o04-133 <jats:p>Zinc is an essential trace element that serves as a structural constituent of a large number of transcription factors, which explains its pivotal role in the control of gene expression. Previous studies investigating the effect of zinc deficiency and zinc supplementation on gene expression in the human adenocarcinoma cell line HT-29 led to the identification of a considerable number of genes responding to alterations in cellular zinc status with changes in steady state mRNA levels. For 9 of 20 genes from these previous screenings that were studied in more detail, mRNA steady state levels responded to both high and low media zinc concentrations. As they are primarily zinc-dependent, we assessed whether these genes are controlled by the zinc-finger metal transcription factor MTF-1. To test this hypothesis we generated a doxycyline-inducible Tet-On HT-29 cell line overexpressing MTF-1. Using this conditional expression system, we present evidence that Kruppel-like factor 4 (klf4), hepatitis A virus cellular receptor 1 (hhav), and complement factor B (cfbp) are 3 potential new target genes of MTF-1. To support this, we used in silico analysis to screen for metal-responsive elements (MREs) within promotors of zinc-sensitive genes. We conclude that zinc responsiveness of klf4, hhav, and cfbp in HT-29 cells is mediated at least in part by MTF-1.Key words: zinc-sensitive genes, target genes, MTF-1, HT-29 cells, metal-response element.</jats:p> Zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (MTF-1) Biochemistry and Cell Biology |
| spellingShingle | Kindermann, Birgit, Döring, Frank, Budczies, Jan, Daniel, Hannelore, Biochemistry and Cell Biology, Zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (MTF-1), Cell Biology, Molecular Biology, Biochemistry |
| title | Zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (MTF-1) |
| title_full | Zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (MTF-1) |
| title_fullStr | Zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (MTF-1) |
| title_full_unstemmed | Zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (MTF-1) |
| title_short | Zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (MTF-1) |
| title_sort | zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (mtf-1) |
| title_unstemmed | Zinc-sensitive genes as potential new target genes of the metal transcription factor-1 (MTF-1) |
| topic | Cell Biology, Molecular Biology, Biochemistry |
| url | http://dx.doi.org/10.1139/o04-133 |