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Preconditioning with morphine protects hippocampal CA1 neurons from ischemia–reperfusion injury via activation of the mTOR pathway
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Zeitschriftentitel: | Canadian Journal of Physiology and Pharmacology |
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Personen und Körperschaften: | , , , , , , |
In: | Canadian Journal of Physiology and Pharmacology, 96, 2018, 1, S. 80-87 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
Canadian Science Publishing
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Schlagwörter: |
author_facet |
Arabian, Maedeh Aboutaleb, Nahid Soleimani, Mansoureh Ajami, Marjan Habibey, Rouhollah Rezaei, Yousef Pazoki-Toroudi, Hamidreza Arabian, Maedeh Aboutaleb, Nahid Soleimani, Mansoureh Ajami, Marjan Habibey, Rouhollah Rezaei, Yousef Pazoki-Toroudi, Hamidreza |
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author |
Arabian, Maedeh Aboutaleb, Nahid Soleimani, Mansoureh Ajami, Marjan Habibey, Rouhollah Rezaei, Yousef Pazoki-Toroudi, Hamidreza |
spellingShingle |
Arabian, Maedeh Aboutaleb, Nahid Soleimani, Mansoureh Ajami, Marjan Habibey, Rouhollah Rezaei, Yousef Pazoki-Toroudi, Hamidreza Canadian Journal of Physiology and Pharmacology Preconditioning with morphine protects hippocampal CA1 neurons from ischemia–reperfusion injury via activation of the mTOR pathway Physiology (medical) Pharmacology General Medicine Physiology |
author_sort |
arabian, maedeh |
spelling |
Arabian, Maedeh Aboutaleb, Nahid Soleimani, Mansoureh Ajami, Marjan Habibey, Rouhollah Rezaei, Yousef Pazoki-Toroudi, Hamidreza 0008-4212 1205-7541 Canadian Science Publishing Physiology (medical) Pharmacology General Medicine Physiology http://dx.doi.org/10.1139/cjpp-2017-0245 <jats:p> The signaling pathway of chronic morphine treatment to prevent neuronal damage following transient cerebral ischemia is not clear. In this study, we examined the role of mammalian target of rapamycin (mTOR) to identify the neuroprotective effects of chronic morphine preconditioning on the hippocampus following ischemia–reperfusion (I/R) injury. Morphine was administered for 5 days, twice a day, before inducing I/R injury. The possible role of mTOR was evaluated by the injection of rapamycin (5 mg/kg body weight, by intraperitoneal injection) before I/R was induced. The passive avoidance test was used to evaluate memory performance. Neuronal density and apoptosis were measured in the CA1 region, 72 h after I/R injury. The expressions of mTOR and phosphorylated mTOR (p-mTOR), as well as superoxide dismutase (SOD) activity were determined 24 h after I/R injury. Chronic morphine treatment attenuated apoptosis and neuronal loss in the hippocampus after I/R injury, which led to improvement in memory (P < 0.05 vs. untreated I/R) and increase in the expression of p-mTOR (P < 0.05 vs. untreated I/R) and SOD activity (P < 0.05 vs. untreated I/R) in the hippocampus. Pretreatment with rapamycin abolished all the above-mentioned protective effects. These results describe novel findings whereby chronic morphine preconditioning in hippocampal CA1 neurons is mediated by the mTOR pathway, and through increased phosphorylation of mTOR can alleviate oxidative stress and apoptosis, and eventually protect the hippocampus from I/R injury. </jats:p> Preconditioning with morphine protects hippocampal CA1 neurons from ischemia–reperfusion injury via activation of the mTOR pathway Canadian Journal of Physiology and Pharmacology |
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10.1139/cjpp-2017-0245 |
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Biologie Chemie und Pharmazie |
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title |
Preconditioning with morphine protects hippocampal CA1 neurons from ischemia–reperfusion injury via activation of the mTOR pathway |
title_unstemmed |
Preconditioning with morphine protects hippocampal CA1 neurons from ischemia–reperfusion injury via activation of the mTOR pathway |
title_full |
Preconditioning with morphine protects hippocampal CA1 neurons from ischemia–reperfusion injury via activation of the mTOR pathway |
title_fullStr |
Preconditioning with morphine protects hippocampal CA1 neurons from ischemia–reperfusion injury via activation of the mTOR pathway |
title_full_unstemmed |
Preconditioning with morphine protects hippocampal CA1 neurons from ischemia–reperfusion injury via activation of the mTOR pathway |
title_short |
Preconditioning with morphine protects hippocampal CA1 neurons from ischemia–reperfusion injury via activation of the mTOR pathway |
title_sort |
preconditioning with morphine protects hippocampal ca1 neurons from ischemia–reperfusion injury via activation of the mtor pathway |
topic |
Physiology (medical) Pharmacology General Medicine Physiology |
url |
http://dx.doi.org/10.1139/cjpp-2017-0245 |
publishDate |
2018 |
physical |
80-87 |
description |
<jats:p> The signaling pathway of chronic morphine treatment to prevent neuronal damage following transient cerebral ischemia is not clear. In this study, we examined the role of mammalian target of rapamycin (mTOR) to identify the neuroprotective effects of chronic morphine preconditioning on the hippocampus following ischemia–reperfusion (I/R) injury. Morphine was administered for 5 days, twice a day, before inducing I/R injury. The possible role of mTOR was evaluated by the injection of rapamycin (5 mg/kg body weight, by intraperitoneal injection) before I/R was induced. The passive avoidance test was used to evaluate memory performance. Neuronal density and apoptosis were measured in the CA1 region, 72 h after I/R injury. The expressions of mTOR and phosphorylated mTOR (p-mTOR), as well as superoxide dismutase (SOD) activity were determined 24 h after I/R injury. Chronic morphine treatment attenuated apoptosis and neuronal loss in the hippocampus after I/R injury, which led to improvement in memory (P < 0.05 vs. untreated I/R) and increase in the expression of p-mTOR (P < 0.05 vs. untreated I/R) and SOD activity (P < 0.05 vs. untreated I/R) in the hippocampus. Pretreatment with rapamycin abolished all the above-mentioned protective effects. These results describe novel findings whereby chronic morphine preconditioning in hippocampal CA1 neurons is mediated by the mTOR pathway, and through increased phosphorylation of mTOR can alleviate oxidative stress and apoptosis, and eventually protect the hippocampus from I/R injury. </jats:p> |
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author | Arabian, Maedeh, Aboutaleb, Nahid, Soleimani, Mansoureh, Ajami, Marjan, Habibey, Rouhollah, Rezaei, Yousef, Pazoki-Toroudi, Hamidreza |
author_facet | Arabian, Maedeh, Aboutaleb, Nahid, Soleimani, Mansoureh, Ajami, Marjan, Habibey, Rouhollah, Rezaei, Yousef, Pazoki-Toroudi, Hamidreza, Arabian, Maedeh, Aboutaleb, Nahid, Soleimani, Mansoureh, Ajami, Marjan, Habibey, Rouhollah, Rezaei, Yousef, Pazoki-Toroudi, Hamidreza |
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description | <jats:p> The signaling pathway of chronic morphine treatment to prevent neuronal damage following transient cerebral ischemia is not clear. In this study, we examined the role of mammalian target of rapamycin (mTOR) to identify the neuroprotective effects of chronic morphine preconditioning on the hippocampus following ischemia–reperfusion (I/R) injury. Morphine was administered for 5 days, twice a day, before inducing I/R injury. The possible role of mTOR was evaluated by the injection of rapamycin (5 mg/kg body weight, by intraperitoneal injection) before I/R was induced. The passive avoidance test was used to evaluate memory performance. Neuronal density and apoptosis were measured in the CA1 region, 72 h after I/R injury. The expressions of mTOR and phosphorylated mTOR (p-mTOR), as well as superoxide dismutase (SOD) activity were determined 24 h after I/R injury. Chronic morphine treatment attenuated apoptosis and neuronal loss in the hippocampus after I/R injury, which led to improvement in memory (P < 0.05 vs. untreated I/R) and increase in the expression of p-mTOR (P < 0.05 vs. untreated I/R) and SOD activity (P < 0.05 vs. untreated I/R) in the hippocampus. Pretreatment with rapamycin abolished all the above-mentioned protective effects. These results describe novel findings whereby chronic morphine preconditioning in hippocampal CA1 neurons is mediated by the mTOR pathway, and through increased phosphorylation of mTOR can alleviate oxidative stress and apoptosis, and eventually protect the hippocampus from I/R injury. </jats:p> |
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spelling | Arabian, Maedeh Aboutaleb, Nahid Soleimani, Mansoureh Ajami, Marjan Habibey, Rouhollah Rezaei, Yousef Pazoki-Toroudi, Hamidreza 0008-4212 1205-7541 Canadian Science Publishing Physiology (medical) Pharmacology General Medicine Physiology http://dx.doi.org/10.1139/cjpp-2017-0245 <jats:p> The signaling pathway of chronic morphine treatment to prevent neuronal damage following transient cerebral ischemia is not clear. In this study, we examined the role of mammalian target of rapamycin (mTOR) to identify the neuroprotective effects of chronic morphine preconditioning on the hippocampus following ischemia–reperfusion (I/R) injury. Morphine was administered for 5 days, twice a day, before inducing I/R injury. The possible role of mTOR was evaluated by the injection of rapamycin (5 mg/kg body weight, by intraperitoneal injection) before I/R was induced. The passive avoidance test was used to evaluate memory performance. Neuronal density and apoptosis were measured in the CA1 region, 72 h after I/R injury. The expressions of mTOR and phosphorylated mTOR (p-mTOR), as well as superoxide dismutase (SOD) activity were determined 24 h after I/R injury. Chronic morphine treatment attenuated apoptosis and neuronal loss in the hippocampus after I/R injury, which led to improvement in memory (P < 0.05 vs. untreated I/R) and increase in the expression of p-mTOR (P < 0.05 vs. untreated I/R) and SOD activity (P < 0.05 vs. untreated I/R) in the hippocampus. Pretreatment with rapamycin abolished all the above-mentioned protective effects. These results describe novel findings whereby chronic morphine preconditioning in hippocampal CA1 neurons is mediated by the mTOR pathway, and through increased phosphorylation of mTOR can alleviate oxidative stress and apoptosis, and eventually protect the hippocampus from I/R injury. </jats:p> Preconditioning with morphine protects hippocampal CA1 neurons from ischemia–reperfusion injury via activation of the mTOR pathway Canadian Journal of Physiology and Pharmacology |
spellingShingle | Arabian, Maedeh, Aboutaleb, Nahid, Soleimani, Mansoureh, Ajami, Marjan, Habibey, Rouhollah, Rezaei, Yousef, Pazoki-Toroudi, Hamidreza, Canadian Journal of Physiology and Pharmacology, Preconditioning with morphine protects hippocampal CA1 neurons from ischemia–reperfusion injury via activation of the mTOR pathway, Physiology (medical), Pharmacology, General Medicine, Physiology |
title | Preconditioning with morphine protects hippocampal CA1 neurons from ischemia–reperfusion injury via activation of the mTOR pathway |
title_full | Preconditioning with morphine protects hippocampal CA1 neurons from ischemia–reperfusion injury via activation of the mTOR pathway |
title_fullStr | Preconditioning with morphine protects hippocampal CA1 neurons from ischemia–reperfusion injury via activation of the mTOR pathway |
title_full_unstemmed | Preconditioning with morphine protects hippocampal CA1 neurons from ischemia–reperfusion injury via activation of the mTOR pathway |
title_short | Preconditioning with morphine protects hippocampal CA1 neurons from ischemia–reperfusion injury via activation of the mTOR pathway |
title_sort | preconditioning with morphine protects hippocampal ca1 neurons from ischemia–reperfusion injury via activation of the mtor pathway |
title_unstemmed | Preconditioning with morphine protects hippocampal CA1 neurons from ischemia–reperfusion injury via activation of the mTOR pathway |
topic | Physiology (medical), Pharmacology, General Medicine, Physiology |
url | http://dx.doi.org/10.1139/cjpp-2017-0245 |