author_facet Dijkhuizen, Laura W.
Brouwer, Paul
Bolhuis, Henk
Reichart, Gert‐Jan
Koppers, Nils
Huettel, Bruno
Bolger, Anthony M.
Li, Fay‐Wei
Cheng, Shifeng
Liu, Xin
Wong, Gane Ka‐Shu
Pryer, Kathleen
Weber, Andreas
Bräutigam, Andrea
Schluepmann, Henriette
Dijkhuizen, Laura W.
Brouwer, Paul
Bolhuis, Henk
Reichart, Gert‐Jan
Koppers, Nils
Huettel, Bruno
Bolger, Anthony M.
Li, Fay‐Wei
Cheng, Shifeng
Liu, Xin
Wong, Gane Ka‐Shu
Pryer, Kathleen
Weber, Andreas
Bräutigam, Andrea
Schluepmann, Henriette
author Dijkhuizen, Laura W.
Brouwer, Paul
Bolhuis, Henk
Reichart, Gert‐Jan
Koppers, Nils
Huettel, Bruno
Bolger, Anthony M.
Li, Fay‐Wei
Cheng, Shifeng
Liu, Xin
Wong, Gane Ka‐Shu
Pryer, Kathleen
Weber, Andreas
Bräutigam, Andrea
Schluepmann, Henriette
spellingShingle Dijkhuizen, Laura W.
Brouwer, Paul
Bolhuis, Henk
Reichart, Gert‐Jan
Koppers, Nils
Huettel, Bruno
Bolger, Anthony M.
Li, Fay‐Wei
Cheng, Shifeng
Liu, Xin
Wong, Gane Ka‐Shu
Pryer, Kathleen
Weber, Andreas
Bräutigam, Andrea
Schluepmann, Henriette
New Phytologist
Is there foul play in the leaf pocket? The metagenome of floating fern Azolla reveals endophytes that do not fix N2 but may denitrify
Plant Science
Physiology
author_sort dijkhuizen, laura w.
spelling Dijkhuizen, Laura W. Brouwer, Paul Bolhuis, Henk Reichart, Gert‐Jan Koppers, Nils Huettel, Bruno Bolger, Anthony M. Li, Fay‐Wei Cheng, Shifeng Liu, Xin Wong, Gane Ka‐Shu Pryer, Kathleen Weber, Andreas Bräutigam, Andrea Schluepmann, Henriette 0028-646X 1469-8137 Wiley Plant Science Physiology http://dx.doi.org/10.1111/nph.14843 <jats:title>Summary</jats:title><jats:p> <jats:list list-type="bullet"> <jats:list-item><jats:p>Dinitrogen fixation by <jats:italic>Nostoc azollae</jats:italic> residing in specialized leaf pockets supports prolific growth of the floating fern <jats:italic>Azolla filiculoides</jats:italic>. To evaluate contributions by further microorganisms, the <jats:italic>A. filiculoides</jats:italic> microbiome and nitrogen metabolism in bacteria persistently associated with <jats:italic>Azolla</jats:italic> ferns were characterized.</jats:p></jats:list-item> <jats:list-item><jats:p>A metagenomic approach was taken complemented by detection of N<jats:sub>2</jats:sub>O released and nitrogen isotope determinations of fern biomass. Ribosomal RNA genes in sequenced DNA of natural ferns, their enriched leaf pockets and water filtrate from the surrounding ditch established that bacteria of <jats:italic>A. filiculoides</jats:italic> differed entirely from surrounding water and revealed species of the order Rhizobiales. Analyses of seven cultivated <jats:italic>Azolla</jats:italic> species confirmed persistent association with Rhizobiales.</jats:p></jats:list-item> <jats:list-item><jats:p>Two distinct nearly full‐length Rhizobiales genomes were identified in leaf‐pocket‐enriched samples from ditch grown <jats:italic>A</jats:italic>. <jats:italic>filiculoides</jats:italic>. Their annotation revealed genes for denitrification but not N<jats:sub>2</jats:sub>‐fixation. <jats:sup>15</jats:sup>N<jats:sub>2</jats:sub> incorporation was active in ferns with <jats:italic>N. azollae</jats:italic> but not in ferns without. N<jats:sub>2</jats:sub>O was not detectably released from surface‐sterilized ferns with the Rhizobiales.</jats:p></jats:list-item> <jats:list-item><jats:p>N<jats:sub>2</jats:sub>‐fixing <jats:italic>N. azollae</jats:italic>, we conclude, dominated the microbiome of <jats:italic>Azolla</jats:italic> ferns. The persistent but less abundant heterotrophic Rhizobiales bacteria possibly contributed to lowering O<jats:sub>2</jats:sub> levels in leaf pockets but did not release detectable amounts of the strong greenhouse gas N<jats:sub>2</jats:sub>O.</jats:p></jats:list-item> </jats:list> </jats:p> Is there foul play in the leaf pocket? The metagenome of floating fern <i>Azolla</i> reveals endophytes that do not fix N<sub>2</sub> but may denitrify New Phytologist
doi_str_mv 10.1111/nph.14843
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recordtype ai
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series New Phytologist
source_id 49
title Is there foul play in the leaf pocket? The metagenome of floating fern Azolla reveals endophytes that do not fix N2 but may denitrify
title_unstemmed Is there foul play in the leaf pocket? The metagenome of floating fern Azolla reveals endophytes that do not fix N2 but may denitrify
title_full Is there foul play in the leaf pocket? The metagenome of floating fern Azolla reveals endophytes that do not fix N2 but may denitrify
title_fullStr Is there foul play in the leaf pocket? The metagenome of floating fern Azolla reveals endophytes that do not fix N2 but may denitrify
title_full_unstemmed Is there foul play in the leaf pocket? The metagenome of floating fern Azolla reveals endophytes that do not fix N2 but may denitrify
title_short Is there foul play in the leaf pocket? The metagenome of floating fern Azolla reveals endophytes that do not fix N2 but may denitrify
title_sort is there foul play in the leaf pocket? the metagenome of floating fern <i>azolla</i> reveals endophytes that do not fix n<sub>2</sub> but may denitrify
topic Plant Science
Physiology
url http://dx.doi.org/10.1111/nph.14843
publishDate 2018
physical 453-466
description <jats:title>Summary</jats:title><jats:p> <jats:list list-type="bullet"> <jats:list-item><jats:p>Dinitrogen fixation by <jats:italic>Nostoc azollae</jats:italic> residing in specialized leaf pockets supports prolific growth of the floating fern <jats:italic>Azolla filiculoides</jats:italic>. To evaluate contributions by further microorganisms, the <jats:italic>A. filiculoides</jats:italic> microbiome and nitrogen metabolism in bacteria persistently associated with <jats:italic>Azolla</jats:italic> ferns were characterized.</jats:p></jats:list-item> <jats:list-item><jats:p>A metagenomic approach was taken complemented by detection of N<jats:sub>2</jats:sub>O released and nitrogen isotope determinations of fern biomass. Ribosomal RNA genes in sequenced DNA of natural ferns, their enriched leaf pockets and water filtrate from the surrounding ditch established that bacteria of <jats:italic>A. filiculoides</jats:italic> differed entirely from surrounding water and revealed species of the order Rhizobiales. Analyses of seven cultivated <jats:italic>Azolla</jats:italic> species confirmed persistent association with Rhizobiales.</jats:p></jats:list-item> <jats:list-item><jats:p>Two distinct nearly full‐length Rhizobiales genomes were identified in leaf‐pocket‐enriched samples from ditch grown <jats:italic>A</jats:italic>. <jats:italic>filiculoides</jats:italic>. Their annotation revealed genes for denitrification but not N<jats:sub>2</jats:sub>‐fixation. <jats:sup>15</jats:sup>N<jats:sub>2</jats:sub> incorporation was active in ferns with <jats:italic>N. azollae</jats:italic> but not in ferns without. N<jats:sub>2</jats:sub>O was not detectably released from surface‐sterilized ferns with the Rhizobiales.</jats:p></jats:list-item> <jats:list-item><jats:p>N<jats:sub>2</jats:sub>‐fixing <jats:italic>N. azollae</jats:italic>, we conclude, dominated the microbiome of <jats:italic>Azolla</jats:italic> ferns. The persistent but less abundant heterotrophic Rhizobiales bacteria possibly contributed to lowering O<jats:sub>2</jats:sub> levels in leaf pockets but did not release detectable amounts of the strong greenhouse gas N<jats:sub>2</jats:sub>O.</jats:p></jats:list-item> </jats:list> </jats:p>
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author Dijkhuizen, Laura W., Brouwer, Paul, Bolhuis, Henk, Reichart, Gert‐Jan, Koppers, Nils, Huettel, Bruno, Bolger, Anthony M., Li, Fay‐Wei, Cheng, Shifeng, Liu, Xin, Wong, Gane Ka‐Shu, Pryer, Kathleen, Weber, Andreas, Bräutigam, Andrea, Schluepmann, Henriette
author_facet Dijkhuizen, Laura W., Brouwer, Paul, Bolhuis, Henk, Reichart, Gert‐Jan, Koppers, Nils, Huettel, Bruno, Bolger, Anthony M., Li, Fay‐Wei, Cheng, Shifeng, Liu, Xin, Wong, Gane Ka‐Shu, Pryer, Kathleen, Weber, Andreas, Bräutigam, Andrea, Schluepmann, Henriette, Dijkhuizen, Laura W., Brouwer, Paul, Bolhuis, Henk, Reichart, Gert‐Jan, Koppers, Nils, Huettel, Bruno, Bolger, Anthony M., Li, Fay‐Wei, Cheng, Shifeng, Liu, Xin, Wong, Gane Ka‐Shu, Pryer, Kathleen, Weber, Andreas, Bräutigam, Andrea, Schluepmann, Henriette
author_sort dijkhuizen, laura w.
container_issue 1
container_start_page 453
container_title New Phytologist
container_volume 217
description <jats:title>Summary</jats:title><jats:p> <jats:list list-type="bullet"> <jats:list-item><jats:p>Dinitrogen fixation by <jats:italic>Nostoc azollae</jats:italic> residing in specialized leaf pockets supports prolific growth of the floating fern <jats:italic>Azolla filiculoides</jats:italic>. To evaluate contributions by further microorganisms, the <jats:italic>A. filiculoides</jats:italic> microbiome and nitrogen metabolism in bacteria persistently associated with <jats:italic>Azolla</jats:italic> ferns were characterized.</jats:p></jats:list-item> <jats:list-item><jats:p>A metagenomic approach was taken complemented by detection of N<jats:sub>2</jats:sub>O released and nitrogen isotope determinations of fern biomass. Ribosomal RNA genes in sequenced DNA of natural ferns, their enriched leaf pockets and water filtrate from the surrounding ditch established that bacteria of <jats:italic>A. filiculoides</jats:italic> differed entirely from surrounding water and revealed species of the order Rhizobiales. Analyses of seven cultivated <jats:italic>Azolla</jats:italic> species confirmed persistent association with Rhizobiales.</jats:p></jats:list-item> <jats:list-item><jats:p>Two distinct nearly full‐length Rhizobiales genomes were identified in leaf‐pocket‐enriched samples from ditch grown <jats:italic>A</jats:italic>. <jats:italic>filiculoides</jats:italic>. Their annotation revealed genes for denitrification but not N<jats:sub>2</jats:sub>‐fixation. <jats:sup>15</jats:sup>N<jats:sub>2</jats:sub> incorporation was active in ferns with <jats:italic>N. azollae</jats:italic> but not in ferns without. N<jats:sub>2</jats:sub>O was not detectably released from surface‐sterilized ferns with the Rhizobiales.</jats:p></jats:list-item> <jats:list-item><jats:p>N<jats:sub>2</jats:sub>‐fixing <jats:italic>N. azollae</jats:italic>, we conclude, dominated the microbiome of <jats:italic>Azolla</jats:italic> ferns. The persistent but less abundant heterotrophic Rhizobiales bacteria possibly contributed to lowering O<jats:sub>2</jats:sub> levels in leaf pockets but did not release detectable amounts of the strong greenhouse gas N<jats:sub>2</jats:sub>O.</jats:p></jats:list-item> </jats:list> </jats:p>
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spelling Dijkhuizen, Laura W. Brouwer, Paul Bolhuis, Henk Reichart, Gert‐Jan Koppers, Nils Huettel, Bruno Bolger, Anthony M. Li, Fay‐Wei Cheng, Shifeng Liu, Xin Wong, Gane Ka‐Shu Pryer, Kathleen Weber, Andreas Bräutigam, Andrea Schluepmann, Henriette 0028-646X 1469-8137 Wiley Plant Science Physiology http://dx.doi.org/10.1111/nph.14843 <jats:title>Summary</jats:title><jats:p> <jats:list list-type="bullet"> <jats:list-item><jats:p>Dinitrogen fixation by <jats:italic>Nostoc azollae</jats:italic> residing in specialized leaf pockets supports prolific growth of the floating fern <jats:italic>Azolla filiculoides</jats:italic>. To evaluate contributions by further microorganisms, the <jats:italic>A. filiculoides</jats:italic> microbiome and nitrogen metabolism in bacteria persistently associated with <jats:italic>Azolla</jats:italic> ferns were characterized.</jats:p></jats:list-item> <jats:list-item><jats:p>A metagenomic approach was taken complemented by detection of N<jats:sub>2</jats:sub>O released and nitrogen isotope determinations of fern biomass. Ribosomal RNA genes in sequenced DNA of natural ferns, their enriched leaf pockets and water filtrate from the surrounding ditch established that bacteria of <jats:italic>A. filiculoides</jats:italic> differed entirely from surrounding water and revealed species of the order Rhizobiales. Analyses of seven cultivated <jats:italic>Azolla</jats:italic> species confirmed persistent association with Rhizobiales.</jats:p></jats:list-item> <jats:list-item><jats:p>Two distinct nearly full‐length Rhizobiales genomes were identified in leaf‐pocket‐enriched samples from ditch grown <jats:italic>A</jats:italic>. <jats:italic>filiculoides</jats:italic>. Their annotation revealed genes for denitrification but not N<jats:sub>2</jats:sub>‐fixation. <jats:sup>15</jats:sup>N<jats:sub>2</jats:sub> incorporation was active in ferns with <jats:italic>N. azollae</jats:italic> but not in ferns without. N<jats:sub>2</jats:sub>O was not detectably released from surface‐sterilized ferns with the Rhizobiales.</jats:p></jats:list-item> <jats:list-item><jats:p>N<jats:sub>2</jats:sub>‐fixing <jats:italic>N. azollae</jats:italic>, we conclude, dominated the microbiome of <jats:italic>Azolla</jats:italic> ferns. The persistent but less abundant heterotrophic Rhizobiales bacteria possibly contributed to lowering O<jats:sub>2</jats:sub> levels in leaf pockets but did not release detectable amounts of the strong greenhouse gas N<jats:sub>2</jats:sub>O.</jats:p></jats:list-item> </jats:list> </jats:p> Is there foul play in the leaf pocket? The metagenome of floating fern <i>Azolla</i> reveals endophytes that do not fix N<sub>2</sub> but may denitrify New Phytologist
spellingShingle Dijkhuizen, Laura W., Brouwer, Paul, Bolhuis, Henk, Reichart, Gert‐Jan, Koppers, Nils, Huettel, Bruno, Bolger, Anthony M., Li, Fay‐Wei, Cheng, Shifeng, Liu, Xin, Wong, Gane Ka‐Shu, Pryer, Kathleen, Weber, Andreas, Bräutigam, Andrea, Schluepmann, Henriette, New Phytologist, Is there foul play in the leaf pocket? The metagenome of floating fern Azolla reveals endophytes that do not fix N2 but may denitrify, Plant Science, Physiology
title Is there foul play in the leaf pocket? The metagenome of floating fern Azolla reveals endophytes that do not fix N2 but may denitrify
title_full Is there foul play in the leaf pocket? The metagenome of floating fern Azolla reveals endophytes that do not fix N2 but may denitrify
title_fullStr Is there foul play in the leaf pocket? The metagenome of floating fern Azolla reveals endophytes that do not fix N2 but may denitrify
title_full_unstemmed Is there foul play in the leaf pocket? The metagenome of floating fern Azolla reveals endophytes that do not fix N2 but may denitrify
title_short Is there foul play in the leaf pocket? The metagenome of floating fern Azolla reveals endophytes that do not fix N2 but may denitrify
title_sort is there foul play in the leaf pocket? the metagenome of floating fern <i>azolla</i> reveals endophytes that do not fix n<sub>2</sub> but may denitrify
title_unstemmed Is there foul play in the leaf pocket? The metagenome of floating fern Azolla reveals endophytes that do not fix N2 but may denitrify
topic Plant Science, Physiology
url http://dx.doi.org/10.1111/nph.14843