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Products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells
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| Zeitschriftentitel: | British Journal of Nutrition |
|---|---|
| Personen und Körperschaften: | , , |
| In: | British Journal of Nutrition, 97, 2007, 5, S. 928-937 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Cambridge University Press (CUP)
|
| Schlagwörter: |
| author_facet |
Sauer, Julia Richter, Konrad Klaus Pool-Zobel, Beatrice Louise Sauer, Julia Richter, Konrad Klaus Pool-Zobel, Beatrice Louise |
|---|---|
| author |
Sauer, Julia Richter, Konrad Klaus Pool-Zobel, Beatrice Louise |
| spellingShingle |
Sauer, Julia Richter, Konrad Klaus Pool-Zobel, Beatrice Louise British Journal of Nutrition Products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells Nutrition and Dietetics Medicine (miscellaneous) |
| author_sort |
sauer, julia |
| spelling |
Sauer, Julia Richter, Konrad Klaus Pool-Zobel, Beatrice Louise 0007-1145 1475-2662 Cambridge University Press (CUP) Nutrition and Dietetics Medicine (miscellaneous) http://dx.doi.org/10.1017/s0007114507666422 <jats:p>Inulin-type fructans are fermented by gut bacteria to yield SCFA, including butyrate which is trophic for colonocytes and induces glutathione<jats:italic>S</jats:italic>-transferases (GST) that detoxify carcinogens. Since little is known on similar effects by complex fermentation samples, we studied related products in non-transformed human colonocytes. Inulin enriched with oligofructose (1 : 1, Synergy1) was fermented with human gut flora. SCFA were quantified and a SCFA mixture was prepared accordingly. Colonocytes were incubated (4–12 h) with the Synergy1 fermentation supernatant (SFS), faeces control, a mixture of the three major SCFA (each 0–15 %, v/v) or butyrate (0–50 m<jats:sc>m</jats:sc>). Metabolic activity was determined to assess trophic effects and cytotoxicity. Expression of ninety-six genes related to biotransformation was studied using cDNA macroarrays. Results on modulated GST were reassessed with real-time PCR and GST activity was measured. Fermentation of inulin resulted in 2–3-fold increases of SCFA. The samples were non-cytotoxic. SFS increased metabolic activity, pointing to trophic effects. The samples modulated gene expression with different response patterns. Key results were that<jats:italic>GSTM2</jats:italic>(2·0-fold) and<jats:italic>GSTM5</jats:italic>(2·2-fold) were enhanced by SFS, whereas the SCFA mixture reduced expression. The faeces control enhanced<jats:italic>GSTA4</jats:italic>(2·0-fold), but reduced<jats:italic>GSTM2</jats:italic>(0·2-fold) and<jats:italic>GSTM5</jats:italic>(0·2-fold). Real-time qPCR confirmed the induction of<jats:italic>GSTM2</jats:italic>and<jats:italic>GSTM5</jats:italic>by SFS and of<jats:italic>GSTA4</jats:italic>and<jats:italic>GSTT2</jats:italic>by butyrate. Activity of GST was not modulated. High concentrations of fermentation products were well tolerated by primary colonocytes, pointing to trophic effects. The induction of GST by the SFS may protect the cells from carcinogenic compounds.</jats:p> Products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells British Journal of Nutrition |
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| title |
Products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells |
| title_unstemmed |
Products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells |
| title_full |
Products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells |
| title_fullStr |
Products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells |
| title_full_unstemmed |
Products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells |
| title_short |
Products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells |
| title_sort |
products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells |
| topic |
Nutrition and Dietetics Medicine (miscellaneous) |
| url |
http://dx.doi.org/10.1017/s0007114507666422 |
| publishDate |
2007 |
| physical |
928-937 |
| description |
<jats:p>Inulin-type fructans are fermented by gut bacteria to yield SCFA, including butyrate which is trophic for colonocytes and induces glutathione<jats:italic>S</jats:italic>-transferases (GST) that detoxify carcinogens. Since little is known on similar effects by complex fermentation samples, we studied related products in non-transformed human colonocytes. Inulin enriched with oligofructose (1 : 1, Synergy1) was fermented with human gut flora. SCFA were quantified and a SCFA mixture was prepared accordingly. Colonocytes were incubated (4–12 h) with the Synergy1 fermentation supernatant (SFS), faeces control, a mixture of the three major SCFA (each 0–15 %, v/v) or butyrate (0–50 m<jats:sc>m</jats:sc>). Metabolic activity was determined to assess trophic effects and cytotoxicity. Expression of ninety-six genes related to biotransformation was studied using cDNA macroarrays. Results on modulated GST were reassessed with real-time PCR and GST activity was measured. Fermentation of inulin resulted in 2–3-fold increases of SCFA. The samples were non-cytotoxic. SFS increased metabolic activity, pointing to trophic effects. The samples modulated gene expression with different response patterns. Key results were that<jats:italic>GSTM2</jats:italic>(2·0-fold) and<jats:italic>GSTM5</jats:italic>(2·2-fold) were enhanced by SFS, whereas the SCFA mixture reduced expression. The faeces control enhanced<jats:italic>GSTA4</jats:italic>(2·0-fold), but reduced<jats:italic>GSTM2</jats:italic>(0·2-fold) and<jats:italic>GSTM5</jats:italic>(0·2-fold). Real-time qPCR confirmed the induction of<jats:italic>GSTM2</jats:italic>and<jats:italic>GSTM5</jats:italic>by SFS and of<jats:italic>GSTA4</jats:italic>and<jats:italic>GSTT2</jats:italic>by butyrate. Activity of GST was not modulated. High concentrations of fermentation products were well tolerated by primary colonocytes, pointing to trophic effects. The induction of GST by the SFS may protect the cells from carcinogenic compounds.</jats:p> |
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| author | Sauer, Julia, Richter, Konrad Klaus, Pool-Zobel, Beatrice Louise |
| author_facet | Sauer, Julia, Richter, Konrad Klaus, Pool-Zobel, Beatrice Louise, Sauer, Julia, Richter, Konrad Klaus, Pool-Zobel, Beatrice Louise |
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| description | <jats:p>Inulin-type fructans are fermented by gut bacteria to yield SCFA, including butyrate which is trophic for colonocytes and induces glutathione<jats:italic>S</jats:italic>-transferases (GST) that detoxify carcinogens. Since little is known on similar effects by complex fermentation samples, we studied related products in non-transformed human colonocytes. Inulin enriched with oligofructose (1 : 1, Synergy1) was fermented with human gut flora. SCFA were quantified and a SCFA mixture was prepared accordingly. Colonocytes were incubated (4–12 h) with the Synergy1 fermentation supernatant (SFS), faeces control, a mixture of the three major SCFA (each 0–15 %, v/v) or butyrate (0–50 m<jats:sc>m</jats:sc>). Metabolic activity was determined to assess trophic effects and cytotoxicity. Expression of ninety-six genes related to biotransformation was studied using cDNA macroarrays. Results on modulated GST were reassessed with real-time PCR and GST activity was measured. Fermentation of inulin resulted in 2–3-fold increases of SCFA. The samples were non-cytotoxic. SFS increased metabolic activity, pointing to trophic effects. The samples modulated gene expression with different response patterns. Key results were that<jats:italic>GSTM2</jats:italic>(2·0-fold) and<jats:italic>GSTM5</jats:italic>(2·2-fold) were enhanced by SFS, whereas the SCFA mixture reduced expression. The faeces control enhanced<jats:italic>GSTA4</jats:italic>(2·0-fold), but reduced<jats:italic>GSTM2</jats:italic>(0·2-fold) and<jats:italic>GSTM5</jats:italic>(0·2-fold). Real-time qPCR confirmed the induction of<jats:italic>GSTM2</jats:italic>and<jats:italic>GSTM5</jats:italic>by SFS and of<jats:italic>GSTA4</jats:italic>and<jats:italic>GSTT2</jats:italic>by butyrate. Activity of GST was not modulated. High concentrations of fermentation products were well tolerated by primary colonocytes, pointing to trophic effects. The induction of GST by the SFS may protect the cells from carcinogenic compounds.</jats:p> |
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| imprint_str_mv | Cambridge University Press (CUP), 2007 |
| institution | DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1 |
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| physical | 928-937 |
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| spelling | Sauer, Julia Richter, Konrad Klaus Pool-Zobel, Beatrice Louise 0007-1145 1475-2662 Cambridge University Press (CUP) Nutrition and Dietetics Medicine (miscellaneous) http://dx.doi.org/10.1017/s0007114507666422 <jats:p>Inulin-type fructans are fermented by gut bacteria to yield SCFA, including butyrate which is trophic for colonocytes and induces glutathione<jats:italic>S</jats:italic>-transferases (GST) that detoxify carcinogens. Since little is known on similar effects by complex fermentation samples, we studied related products in non-transformed human colonocytes. Inulin enriched with oligofructose (1 : 1, Synergy1) was fermented with human gut flora. SCFA were quantified and a SCFA mixture was prepared accordingly. Colonocytes were incubated (4–12 h) with the Synergy1 fermentation supernatant (SFS), faeces control, a mixture of the three major SCFA (each 0–15 %, v/v) or butyrate (0–50 m<jats:sc>m</jats:sc>). Metabolic activity was determined to assess trophic effects and cytotoxicity. Expression of ninety-six genes related to biotransformation was studied using cDNA macroarrays. Results on modulated GST were reassessed with real-time PCR and GST activity was measured. Fermentation of inulin resulted in 2–3-fold increases of SCFA. The samples were non-cytotoxic. SFS increased metabolic activity, pointing to trophic effects. The samples modulated gene expression with different response patterns. Key results were that<jats:italic>GSTM2</jats:italic>(2·0-fold) and<jats:italic>GSTM5</jats:italic>(2·2-fold) were enhanced by SFS, whereas the SCFA mixture reduced expression. The faeces control enhanced<jats:italic>GSTA4</jats:italic>(2·0-fold), but reduced<jats:italic>GSTM2</jats:italic>(0·2-fold) and<jats:italic>GSTM5</jats:italic>(0·2-fold). Real-time qPCR confirmed the induction of<jats:italic>GSTM2</jats:italic>and<jats:italic>GSTM5</jats:italic>by SFS and of<jats:italic>GSTA4</jats:italic>and<jats:italic>GSTT2</jats:italic>by butyrate. Activity of GST was not modulated. High concentrations of fermentation products were well tolerated by primary colonocytes, pointing to trophic effects. The induction of GST by the SFS may protect the cells from carcinogenic compounds.</jats:p> Products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells British Journal of Nutrition |
| spellingShingle | Sauer, Julia, Richter, Konrad Klaus, Pool-Zobel, Beatrice Louise, British Journal of Nutrition, Products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells, Nutrition and Dietetics, Medicine (miscellaneous) |
| title | Products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells |
| title_full | Products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells |
| title_fullStr | Products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells |
| title_full_unstemmed | Products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells |
| title_short | Products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells |
| title_sort | products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells |
| title_unstemmed | Products formed during fermentation of the prebiotic inulin with humangut flora enhance expression of biotransformation genes in human primarycolon cells |
| topic | Nutrition and Dietetics, Medicine (miscellaneous) |
| url | http://dx.doi.org/10.1017/s0007114507666422 |