Eintrag weiter verarbeiten
A modified culture medium for improved isolation of marine vibrios
Gespeichert in:
| Zeitschriftentitel: | MicrobiologyOpen |
|---|---|
| Personen und Körperschaften: | , , , , |
| In: | MicrobiologyOpen, 8, 2019, 9 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Tagliavia, Marcello Salamone, Monica Bennici, Carmelo Quatrini, Paola Cuttitta, Angela Tagliavia, Marcello Salamone, Monica Bennici, Carmelo Quatrini, Paola Cuttitta, Angela |
|---|---|
| author |
Tagliavia, Marcello Salamone, Monica Bennici, Carmelo Quatrini, Paola Cuttitta, Angela |
| spellingShingle |
Tagliavia, Marcello Salamone, Monica Bennici, Carmelo Quatrini, Paola Cuttitta, Angela MicrobiologyOpen A modified culture medium for improved isolation of marine vibrios Microbiology |
| author_sort |
tagliavia, marcello |
| spelling |
Tagliavia, Marcello Salamone, Monica Bennici, Carmelo Quatrini, Paola Cuttitta, Angela 2045-8827 2045-8827 Wiley Microbiology http://dx.doi.org/10.1002/mbo3.835 <jats:title>Abstract</jats:title><jats:p>Marine <jats:italic>Vibrio</jats:italic> members are of great interest for both ecological and biotechnological research, which often relies on their isolation. Whereas many efforts have been made for the detection of food‐borne pathogenic species, much less is known about the performances of standard culture media toward environmental vibrios. We show that the isolation/enumeration of marine vibrios using thiosulfate‐citrate‐bile salts‐sucrose agar (TCBS) as selective medium may be hampered by the variable adaptability of different taxa to the medium, which may result even in isolation failure and/or in substantial total count underestimation. We propose a modified TCBS as isolation medium, adjusted for marine vibrios requirements, which greatly improved their recovery in dilution plate counts, compared with the standard medium. The modified medium offers substantial advantages over TCBS, providing more accurate and likely estimations of the actual presence of vibrios. Modified TCBS allowed the recovery of otherwise undetected vibrios, some of which producing biotechnologically valuable enzymes, thus expanding the isolation power toward potentially new enzyme‐producers <jats:italic>Vibrio</jats:italic> taxa. Moreover, we report a newly designed <jats:italic>Vibrio</jats:italic>‐specific PCR primers pair, targeting a unique <jats:italic>rpoD</jats:italic> sequence, useful for rapid confirmation of isolates as <jats:italic>Vibrio</jats:italic> members and subsequent genetic analyses.</jats:p> A modified culture medium for improved isolation of marine vibrios MicrobiologyOpen |
| doi_str_mv |
10.1002/mbo3.835 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9tYm8zLjgzNQ |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9tYm8zLjgzNQ |
| institution |
DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-Zwi2 DE-D161 DE-Gla1 DE-Zi4 DE-15 DE-Pl11 DE-Rs1 |
| imprint |
Wiley, 2019 |
| imprint_str_mv |
Wiley, 2019 |
| issn |
2045-8827 |
| issn_str_mv |
2045-8827 |
| language |
English |
| mega_collection |
Wiley (CrossRef) |
| match_str |
tagliavia2019amodifiedculturemediumforimprovedisolationofmarinevibrios |
| publishDateSort |
2019 |
| publisher |
Wiley |
| recordtype |
ai |
| record_format |
ai |
| series |
MicrobiologyOpen |
| source_id |
49 |
| title |
A modified culture medium for improved isolation of marine vibrios |
| title_unstemmed |
A modified culture medium for improved isolation of marine vibrios |
| title_full |
A modified culture medium for improved isolation of marine vibrios |
| title_fullStr |
A modified culture medium for improved isolation of marine vibrios |
| title_full_unstemmed |
A modified culture medium for improved isolation of marine vibrios |
| title_short |
A modified culture medium for improved isolation of marine vibrios |
| title_sort |
a modified culture medium for improved isolation of marine vibrios |
| topic |
Microbiology |
| url |
http://dx.doi.org/10.1002/mbo3.835 |
| publishDate |
2019 |
| physical |
|
| description |
<jats:title>Abstract</jats:title><jats:p>Marine <jats:italic>Vibrio</jats:italic> members are of great interest for both ecological and biotechnological research, which often relies on their isolation. Whereas many efforts have been made for the detection of food‐borne pathogenic species, much less is known about the performances of standard culture media toward environmental vibrios. We show that the isolation/enumeration of marine vibrios using thiosulfate‐citrate‐bile salts‐sucrose agar (TCBS) as selective medium may be hampered by the variable adaptability of different taxa to the medium, which may result even in isolation failure and/or in substantial total count underestimation. We propose a modified TCBS as isolation medium, adjusted for marine vibrios requirements, which greatly improved their recovery in dilution plate counts, compared with the standard medium. The modified medium offers substantial advantages over TCBS, providing more accurate and likely estimations of the actual presence of vibrios. Modified TCBS allowed the recovery of otherwise undetected vibrios, some of which producing biotechnologically valuable enzymes, thus expanding the isolation power toward potentially new enzyme‐producers <jats:italic>Vibrio</jats:italic> taxa. Moreover, we report a newly designed <jats:italic>Vibrio</jats:italic>‐specific PCR primers pair, targeting a unique <jats:italic>rpoD</jats:italic> sequence, useful for rapid confirmation of isolates as <jats:italic>Vibrio</jats:italic> members and subsequent genetic analyses.</jats:p> |
| container_issue |
9 |
| container_start_page |
0 |
| container_title |
MicrobiologyOpen |
| container_volume |
8 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792345601468268549 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T17:26:03.898Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=A+modified+culture+medium+for+improved+isolation+of+marine+vibrios&rft.date=2019-09-01&genre=article&issn=2045-8827&volume=8&issue=9&jtitle=MicrobiologyOpen&atitle=A+modified+culture+medium+for+improved+isolation+of+marine+vibrios&aulast=Cuttitta&aufirst=Angela&rft_id=info%3Adoi%2F10.1002%2Fmbo3.835&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792345601468268549 |
| author | Tagliavia, Marcello, Salamone, Monica, Bennici, Carmelo, Quatrini, Paola, Cuttitta, Angela |
| author_facet | Tagliavia, Marcello, Salamone, Monica, Bennici, Carmelo, Quatrini, Paola, Cuttitta, Angela, Tagliavia, Marcello, Salamone, Monica, Bennici, Carmelo, Quatrini, Paola, Cuttitta, Angela |
| author_sort | tagliavia, marcello |
| container_issue | 9 |
| container_start_page | 0 |
| container_title | MicrobiologyOpen |
| container_volume | 8 |
| description | <jats:title>Abstract</jats:title><jats:p>Marine <jats:italic>Vibrio</jats:italic> members are of great interest for both ecological and biotechnological research, which often relies on their isolation. Whereas many efforts have been made for the detection of food‐borne pathogenic species, much less is known about the performances of standard culture media toward environmental vibrios. We show that the isolation/enumeration of marine vibrios using thiosulfate‐citrate‐bile salts‐sucrose agar (TCBS) as selective medium may be hampered by the variable adaptability of different taxa to the medium, which may result even in isolation failure and/or in substantial total count underestimation. We propose a modified TCBS as isolation medium, adjusted for marine vibrios requirements, which greatly improved their recovery in dilution plate counts, compared with the standard medium. The modified medium offers substantial advantages over TCBS, providing more accurate and likely estimations of the actual presence of vibrios. Modified TCBS allowed the recovery of otherwise undetected vibrios, some of which producing biotechnologically valuable enzymes, thus expanding the isolation power toward potentially new enzyme‐producers <jats:italic>Vibrio</jats:italic> taxa. Moreover, we report a newly designed <jats:italic>Vibrio</jats:italic>‐specific PCR primers pair, targeting a unique <jats:italic>rpoD</jats:italic> sequence, useful for rapid confirmation of isolates as <jats:italic>Vibrio</jats:italic> members and subsequent genetic analyses.</jats:p> |
| doi_str_mv | 10.1002/mbo3.835 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9tYm8zLjgzNQ |
| imprint | Wiley, 2019 |
| imprint_str_mv | Wiley, 2019 |
| institution | DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1 |
| issn | 2045-8827 |
| issn_str_mv | 2045-8827 |
| language | English |
| last_indexed | 2024-03-01T17:26:03.898Z |
| match_str | tagliavia2019amodifiedculturemediumforimprovedisolationofmarinevibrios |
| mega_collection | Wiley (CrossRef) |
| physical | |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher | Wiley |
| record_format | ai |
| recordtype | ai |
| series | MicrobiologyOpen |
| source_id | 49 |
| spelling | Tagliavia, Marcello Salamone, Monica Bennici, Carmelo Quatrini, Paola Cuttitta, Angela 2045-8827 2045-8827 Wiley Microbiology http://dx.doi.org/10.1002/mbo3.835 <jats:title>Abstract</jats:title><jats:p>Marine <jats:italic>Vibrio</jats:italic> members are of great interest for both ecological and biotechnological research, which often relies on their isolation. Whereas many efforts have been made for the detection of food‐borne pathogenic species, much less is known about the performances of standard culture media toward environmental vibrios. We show that the isolation/enumeration of marine vibrios using thiosulfate‐citrate‐bile salts‐sucrose agar (TCBS) as selective medium may be hampered by the variable adaptability of different taxa to the medium, which may result even in isolation failure and/or in substantial total count underestimation. We propose a modified TCBS as isolation medium, adjusted for marine vibrios requirements, which greatly improved their recovery in dilution plate counts, compared with the standard medium. The modified medium offers substantial advantages over TCBS, providing more accurate and likely estimations of the actual presence of vibrios. Modified TCBS allowed the recovery of otherwise undetected vibrios, some of which producing biotechnologically valuable enzymes, thus expanding the isolation power toward potentially new enzyme‐producers <jats:italic>Vibrio</jats:italic> taxa. Moreover, we report a newly designed <jats:italic>Vibrio</jats:italic>‐specific PCR primers pair, targeting a unique <jats:italic>rpoD</jats:italic> sequence, useful for rapid confirmation of isolates as <jats:italic>Vibrio</jats:italic> members and subsequent genetic analyses.</jats:p> A modified culture medium for improved isolation of marine vibrios MicrobiologyOpen |
| spellingShingle | Tagliavia, Marcello, Salamone, Monica, Bennici, Carmelo, Quatrini, Paola, Cuttitta, Angela, MicrobiologyOpen, A modified culture medium for improved isolation of marine vibrios, Microbiology |
| title | A modified culture medium for improved isolation of marine vibrios |
| title_full | A modified culture medium for improved isolation of marine vibrios |
| title_fullStr | A modified culture medium for improved isolation of marine vibrios |
| title_full_unstemmed | A modified culture medium for improved isolation of marine vibrios |
| title_short | A modified culture medium for improved isolation of marine vibrios |
| title_sort | a modified culture medium for improved isolation of marine vibrios |
| title_unstemmed | A modified culture medium for improved isolation of marine vibrios |
| topic | Microbiology |
| url | http://dx.doi.org/10.1002/mbo3.835 |