author_facet Winkelmann, Isabel
Näßl, Anna‐Maria
Daniel, Hannelore
Wenzel, Uwe
Winkelmann, Isabel
Näßl, Anna‐Maria
Daniel, Hannelore
Wenzel, Uwe
author Winkelmann, Isabel
Näßl, Anna‐Maria
Daniel, Hannelore
Wenzel, Uwe
spellingShingle Winkelmann, Isabel
Näßl, Anna‐Maria
Daniel, Hannelore
Wenzel, Uwe
International Journal of Cancer
Proteome response in HT‐29 human colorectal cancer cells to two apoptosis‐inducing compounds with different mode of action
Cancer Research
Oncology
author_sort winkelmann, isabel
spelling Winkelmann, Isabel Näßl, Anna‐Maria Daniel, Hannelore Wenzel, Uwe 0020-7136 1097-0215 Wiley Cancer Research Oncology http://dx.doi.org/10.1002/ijc.23387 <jats:title>Abstract</jats:title><jats:p>Flavone and camptothecin were both shown to potently induce apoptosis in HT‐29 human colon cancer cells. Whereas camptothecin acts on the basis of topoisomerase‐I inhibition, flavone appears to burst mitochondrial production of reactive oxygen species by increasing respiratory chain activity. In our study, we searched for similarities and differences in the proteome response of HT‐29 cells when treated with the two different compounds. The accessible proteome of HT‐29 cells was separated subsequent to the exposure to flavone or camptothecin by 2D‐polyacrylamide‐gel electrophoresis using pH‐gradients between 4 and 7 and 6 and 11 in the first dimension and proteins with changed expression level were identified by peptide mass fingerprints of tryptic digests of the protein spots. Whereas there was a high congruence with regard to the identities of regulated proteins and their grade of regulation, a number of spots changed specifically only in response to either flavone or camptothecin. Nuclear envelope proteins were specifically increased by camptothecin indicating the intervention of this drug with cell division processes. Increased levels of coproporphyrinogen III oxidase, involved in cytochrome synthesis, and ubiquinol‐cytochrome‐c reductase suggest adaptations to flavone in order to enable a higher substrate flux through the respiratory chain. In conclusion, HT‐29 cells respond to camptothecin and flavone with regulations of many proteins in a similar manner suggesting those alterations to be caused by apoptosis induction. Some protein regulations, however, were specific for each compound and point to the mechanism of their action. © 2008 Wiley‐Liss, Inc.</jats:p> Proteome response in HT‐29 human colorectal cancer cells to two apoptosis‐inducing compounds with different mode of action International Journal of Cancer
doi_str_mv 10.1002/ijc.23387
facet_avail Online
Free
finc_class_facet Medizin
format ElectronicArticle
fullrecord blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9pamMuMjMzODc
id ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9pamMuMjMzODc
institution DE-Gla1
DE-Zi4
DE-15
DE-Pl11
DE-Rs1
DE-105
DE-14
DE-Ch1
DE-L229
DE-D275
DE-Bn3
DE-Brt1
DE-Zwi2
DE-D161
imprint Wiley, 2008
imprint_str_mv Wiley, 2008
issn 0020-7136
1097-0215
issn_str_mv 0020-7136
1097-0215
language English
mega_collection Wiley (CrossRef)
match_str winkelmann2008proteomeresponseinht29humancolorectalcancercellstotwoapoptosisinducingcompoundswithdifferentmodeofaction
publishDateSort 2008
publisher Wiley
recordtype ai
record_format ai
series International Journal of Cancer
source_id 49
title Proteome response in HT‐29 human colorectal cancer cells to two apoptosis‐inducing compounds with different mode of action
title_unstemmed Proteome response in HT‐29 human colorectal cancer cells to two apoptosis‐inducing compounds with different mode of action
title_full Proteome response in HT‐29 human colorectal cancer cells to two apoptosis‐inducing compounds with different mode of action
title_fullStr Proteome response in HT‐29 human colorectal cancer cells to two apoptosis‐inducing compounds with different mode of action
title_full_unstemmed Proteome response in HT‐29 human colorectal cancer cells to two apoptosis‐inducing compounds with different mode of action
title_short Proteome response in HT‐29 human colorectal cancer cells to two apoptosis‐inducing compounds with different mode of action
title_sort proteome response in ht‐29 human colorectal cancer cells to two apoptosis‐inducing compounds with different mode of action
topic Cancer Research
Oncology
url http://dx.doi.org/10.1002/ijc.23387
publishDate 2008
physical 2223-2232
description <jats:title>Abstract</jats:title><jats:p>Flavone and camptothecin were both shown to potently induce apoptosis in HT‐29 human colon cancer cells. Whereas camptothecin acts on the basis of topoisomerase‐I inhibition, flavone appears to burst mitochondrial production of reactive oxygen species by increasing respiratory chain activity. In our study, we searched for similarities and differences in the proteome response of HT‐29 cells when treated with the two different compounds. The accessible proteome of HT‐29 cells was separated subsequent to the exposure to flavone or camptothecin by 2D‐polyacrylamide‐gel electrophoresis using pH‐gradients between 4 and 7 and 6 and 11 in the first dimension and proteins with changed expression level were identified by peptide mass fingerprints of tryptic digests of the protein spots. Whereas there was a high congruence with regard to the identities of regulated proteins and their grade of regulation, a number of spots changed specifically only in response to either flavone or camptothecin. Nuclear envelope proteins were specifically increased by camptothecin indicating the intervention of this drug with cell division processes. Increased levels of coproporphyrinogen III oxidase, involved in cytochrome synthesis, and ubiquinol‐cytochrome‐c reductase suggest adaptations to flavone in order to enable a higher substrate flux through the respiratory chain. In conclusion, HT‐29 cells respond to camptothecin and flavone with regulations of many proteins in a similar manner suggesting those alterations to be caused by apoptosis induction. Some protein regulations, however, were specific for each compound and point to the mechanism of their action. © 2008 Wiley‐Liss, Inc.</jats:p>
container_issue 10
container_start_page 2223
container_title International Journal of Cancer
container_volume 122
format_de105 Article, E-Article
format_de14 Article, E-Article
format_de15 Article, E-Article
format_de520 Article, E-Article
format_de540 Article, E-Article
format_dech1 Article, E-Article
format_ded117 Article, E-Article
format_degla1 E-Article
format_del152 Buch
format_del189 Article, E-Article
format_dezi4 Article
format_dezwi2 Article, E-Article
format_finc Article, E-Article
format_nrw Article, E-Article
_version_ 1792336082391531526
geogr_code not assigned
last_indexed 2024-03-01T14:54:45.779Z
geogr_code_person not assigned
openURL url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Proteome+response+in+HT%E2%80%9029+human+colorectal+cancer+cells+to+two+apoptosis%E2%80%90inducing+compounds+with+different+mode+of+action&rft.date=2008-05-15&genre=article&issn=1097-0215&volume=122&issue=10&spage=2223&epage=2232&pages=2223-2232&jtitle=International+Journal+of+Cancer&atitle=Proteome+response+in+HT%E2%80%9029+human+colorectal+cancer+cells+to+two+apoptosis%E2%80%90inducing+compounds+with+different+mode+of+action&aulast=Wenzel&aufirst=Uwe&rft_id=info%3Adoi%2F10.1002%2Fijc.23387&rft.language%5B0%5D=eng
SOLR
_version_ 1792336082391531526
author Winkelmann, Isabel, Näßl, Anna‐Maria, Daniel, Hannelore, Wenzel, Uwe
author_facet Winkelmann, Isabel, Näßl, Anna‐Maria, Daniel, Hannelore, Wenzel, Uwe, Winkelmann, Isabel, Näßl, Anna‐Maria, Daniel, Hannelore, Wenzel, Uwe
author_sort winkelmann, isabel
container_issue 10
container_start_page 2223
container_title International Journal of Cancer
container_volume 122
description <jats:title>Abstract</jats:title><jats:p>Flavone and camptothecin were both shown to potently induce apoptosis in HT‐29 human colon cancer cells. Whereas camptothecin acts on the basis of topoisomerase‐I inhibition, flavone appears to burst mitochondrial production of reactive oxygen species by increasing respiratory chain activity. In our study, we searched for similarities and differences in the proteome response of HT‐29 cells when treated with the two different compounds. The accessible proteome of HT‐29 cells was separated subsequent to the exposure to flavone or camptothecin by 2D‐polyacrylamide‐gel electrophoresis using pH‐gradients between 4 and 7 and 6 and 11 in the first dimension and proteins with changed expression level were identified by peptide mass fingerprints of tryptic digests of the protein spots. Whereas there was a high congruence with regard to the identities of regulated proteins and their grade of regulation, a number of spots changed specifically only in response to either flavone or camptothecin. Nuclear envelope proteins were specifically increased by camptothecin indicating the intervention of this drug with cell division processes. Increased levels of coproporphyrinogen III oxidase, involved in cytochrome synthesis, and ubiquinol‐cytochrome‐c reductase suggest adaptations to flavone in order to enable a higher substrate flux through the respiratory chain. In conclusion, HT‐29 cells respond to camptothecin and flavone with regulations of many proteins in a similar manner suggesting those alterations to be caused by apoptosis induction. Some protein regulations, however, were specific for each compound and point to the mechanism of their action. © 2008 Wiley‐Liss, Inc.</jats:p>
doi_str_mv 10.1002/ijc.23387
facet_avail Online, Free
finc_class_facet Medizin
format ElectronicArticle
format_de105 Article, E-Article
format_de14 Article, E-Article
format_de15 Article, E-Article
format_de520 Article, E-Article
format_de540 Article, E-Article
format_dech1 Article, E-Article
format_ded117 Article, E-Article
format_degla1 E-Article
format_del152 Buch
format_del189 Article, E-Article
format_dezi4 Article
format_dezwi2 Article, E-Article
format_finc Article, E-Article
format_nrw Article, E-Article
geogr_code not assigned
geogr_code_person not assigned
id ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9pamMuMjMzODc
imprint Wiley, 2008
imprint_str_mv Wiley, 2008
institution DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161
issn 0020-7136, 1097-0215
issn_str_mv 0020-7136, 1097-0215
language English
last_indexed 2024-03-01T14:54:45.779Z
match_str winkelmann2008proteomeresponseinht29humancolorectalcancercellstotwoapoptosisinducingcompoundswithdifferentmodeofaction
mega_collection Wiley (CrossRef)
physical 2223-2232
publishDate 2008
publishDateSort 2008
publisher Wiley
record_format ai
recordtype ai
series International Journal of Cancer
source_id 49
spelling Winkelmann, Isabel Näßl, Anna‐Maria Daniel, Hannelore Wenzel, Uwe 0020-7136 1097-0215 Wiley Cancer Research Oncology http://dx.doi.org/10.1002/ijc.23387 <jats:title>Abstract</jats:title><jats:p>Flavone and camptothecin were both shown to potently induce apoptosis in HT‐29 human colon cancer cells. Whereas camptothecin acts on the basis of topoisomerase‐I inhibition, flavone appears to burst mitochondrial production of reactive oxygen species by increasing respiratory chain activity. In our study, we searched for similarities and differences in the proteome response of HT‐29 cells when treated with the two different compounds. The accessible proteome of HT‐29 cells was separated subsequent to the exposure to flavone or camptothecin by 2D‐polyacrylamide‐gel electrophoresis using pH‐gradients between 4 and 7 and 6 and 11 in the first dimension and proteins with changed expression level were identified by peptide mass fingerprints of tryptic digests of the protein spots. Whereas there was a high congruence with regard to the identities of regulated proteins and their grade of regulation, a number of spots changed specifically only in response to either flavone or camptothecin. Nuclear envelope proteins were specifically increased by camptothecin indicating the intervention of this drug with cell division processes. Increased levels of coproporphyrinogen III oxidase, involved in cytochrome synthesis, and ubiquinol‐cytochrome‐c reductase suggest adaptations to flavone in order to enable a higher substrate flux through the respiratory chain. In conclusion, HT‐29 cells respond to camptothecin and flavone with regulations of many proteins in a similar manner suggesting those alterations to be caused by apoptosis induction. Some protein regulations, however, were specific for each compound and point to the mechanism of their action. © 2008 Wiley‐Liss, Inc.</jats:p> Proteome response in HT‐29 human colorectal cancer cells to two apoptosis‐inducing compounds with different mode of action International Journal of Cancer
spellingShingle Winkelmann, Isabel, Näßl, Anna‐Maria, Daniel, Hannelore, Wenzel, Uwe, International Journal of Cancer, Proteome response in HT‐29 human colorectal cancer cells to two apoptosis‐inducing compounds with different mode of action, Cancer Research, Oncology
title Proteome response in HT‐29 human colorectal cancer cells to two apoptosis‐inducing compounds with different mode of action
title_full Proteome response in HT‐29 human colorectal cancer cells to two apoptosis‐inducing compounds with different mode of action
title_fullStr Proteome response in HT‐29 human colorectal cancer cells to two apoptosis‐inducing compounds with different mode of action
title_full_unstemmed Proteome response in HT‐29 human colorectal cancer cells to two apoptosis‐inducing compounds with different mode of action
title_short Proteome response in HT‐29 human colorectal cancer cells to two apoptosis‐inducing compounds with different mode of action
title_sort proteome response in ht‐29 human colorectal cancer cells to two apoptosis‐inducing compounds with different mode of action
title_unstemmed Proteome response in HT‐29 human colorectal cancer cells to two apoptosis‐inducing compounds with different mode of action
topic Cancer Research, Oncology
url http://dx.doi.org/10.1002/ijc.23387