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Photometric evaluation of slab gels II: Delimitation and integration of peaks in one‐dimensional electropherograms
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| Zeitschriftentitel: | ELECTROPHORESIS |
|---|---|
| Personen und Körperschaften: | , , , |
| In: | ELECTROPHORESIS, 3, 1982, 5, S. 244-254 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Yakin, Habib M. Kronberg, Harald Zimmer, Hans‐G. Neuhoff, Volker Yakin, Habib M. Kronberg, Harald Zimmer, Hans‐G. Neuhoff, Volker |
|---|---|
| author |
Yakin, Habib M. Kronberg, Harald Zimmer, Hans‐G. Neuhoff, Volker |
| spellingShingle |
Yakin, Habib M. Kronberg, Harald Zimmer, Hans‐G. Neuhoff, Volker ELECTROPHORESIS Photometric evaluation of slab gels II: Delimitation and integration of peaks in one‐dimensional electropherograms Clinical Biochemistry Biochemistry Analytical Chemistry |
| author_sort |
yakin, habib m. |
| spelling |
Yakin, Habib M. Kronberg, Harald Zimmer, Hans‐G. Neuhoff, Volker 0173-0835 1522-2683 Wiley Clinical Biochemistry Biochemistry Analytical Chemistry http://dx.doi.org/10.1002/elps.1150030503 <jats:title>Abstract</jats:title><jats:p>In order to increase accuracy and precision of quantifying one‐dimensional electropherograms by digital signal processing, a set of algorithms is presented that operate on the optical density values acquired by a linear scanning densitometer. These operations include smoothing of the measuring values, background correction by an optimized piecewise linear baseline, and integration of the peak areas after two‐sided fit of half‐Gaussian profiles to each stained protein band. The algorithms are implemented as BASIC‐programs and are readily adaptable to a variety of electrophoretic separations. The procedure has been applied successfully in research [1] as well as in clinical evaluations [2].</jats:p> Photometric evaluation of slab gels II: Delimitation and integration of peaks in one‐dimensional electropherograms ELECTROPHORESIS |
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Wiley, 1982 |
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Wiley, 1982 |
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1522-2683 0173-0835 |
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1982 |
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ELECTROPHORESIS |
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| title |
Photometric evaluation of slab gels II: Delimitation and integration of peaks in one‐dimensional electropherograms |
| title_unstemmed |
Photometric evaluation of slab gels II: Delimitation and integration of peaks in one‐dimensional electropherograms |
| title_full |
Photometric evaluation of slab gels II: Delimitation and integration of peaks in one‐dimensional electropherograms |
| title_fullStr |
Photometric evaluation of slab gels II: Delimitation and integration of peaks in one‐dimensional electropherograms |
| title_full_unstemmed |
Photometric evaluation of slab gels II: Delimitation and integration of peaks in one‐dimensional electropherograms |
| title_short |
Photometric evaluation of slab gels II: Delimitation and integration of peaks in one‐dimensional electropherograms |
| title_sort |
photometric evaluation of slab gels ii: delimitation and integration of peaks in one‐dimensional electropherograms |
| topic |
Clinical Biochemistry Biochemistry Analytical Chemistry |
| url |
http://dx.doi.org/10.1002/elps.1150030503 |
| publishDate |
1982 |
| physical |
244-254 |
| description |
<jats:title>Abstract</jats:title><jats:p>In order to increase accuracy and precision of quantifying one‐dimensional electropherograms by digital signal processing, a set of algorithms is presented that operate on the optical density values acquired by a linear scanning densitometer. These operations include smoothing of the measuring values, background correction by an optimized piecewise linear baseline, and integration of the peak areas after two‐sided fit of half‐Gaussian profiles to each stained protein band. The algorithms are implemented as BASIC‐programs and are readily adaptable to a variety of electrophoretic separations. The procedure has been applied successfully in research [1] as well as in clinical evaluations [2].</jats:p> |
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| author | Yakin, Habib M., Kronberg, Harald, Zimmer, Hans‐G., Neuhoff, Volker |
| author_facet | Yakin, Habib M., Kronberg, Harald, Zimmer, Hans‐G., Neuhoff, Volker, Yakin, Habib M., Kronberg, Harald, Zimmer, Hans‐G., Neuhoff, Volker |
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| description | <jats:title>Abstract</jats:title><jats:p>In order to increase accuracy and precision of quantifying one‐dimensional electropherograms by digital signal processing, a set of algorithms is presented that operate on the optical density values acquired by a linear scanning densitometer. These operations include smoothing of the measuring values, background correction by an optimized piecewise linear baseline, and integration of the peak areas after two‐sided fit of half‐Gaussian profiles to each stained protein band. The algorithms are implemented as BASIC‐programs and are readily adaptable to a variety of electrophoretic separations. The procedure has been applied successfully in research [1] as well as in clinical evaluations [2].</jats:p> |
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| imprint | Wiley, 1982 |
| imprint_str_mv | Wiley, 1982 |
| institution | DE-Bn3, DE-Brt1, DE-D161, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275 |
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| physical | 244-254 |
| publishDate | 1982 |
| publishDateSort | 1982 |
| publisher | Wiley |
| record_format | ai |
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| series | ELECTROPHORESIS |
| source_id | 49 |
| spelling | Yakin, Habib M. Kronberg, Harald Zimmer, Hans‐G. Neuhoff, Volker 0173-0835 1522-2683 Wiley Clinical Biochemistry Biochemistry Analytical Chemistry http://dx.doi.org/10.1002/elps.1150030503 <jats:title>Abstract</jats:title><jats:p>In order to increase accuracy and precision of quantifying one‐dimensional electropherograms by digital signal processing, a set of algorithms is presented that operate on the optical density values acquired by a linear scanning densitometer. These operations include smoothing of the measuring values, background correction by an optimized piecewise linear baseline, and integration of the peak areas after two‐sided fit of half‐Gaussian profiles to each stained protein band. The algorithms are implemented as BASIC‐programs and are readily adaptable to a variety of electrophoretic separations. The procedure has been applied successfully in research [1] as well as in clinical evaluations [2].</jats:p> Photometric evaluation of slab gels II: Delimitation and integration of peaks in one‐dimensional electropherograms ELECTROPHORESIS |
| spellingShingle | Yakin, Habib M., Kronberg, Harald, Zimmer, Hans‐G., Neuhoff, Volker, ELECTROPHORESIS, Photometric evaluation of slab gels II: Delimitation and integration of peaks in one‐dimensional electropherograms, Clinical Biochemistry, Biochemistry, Analytical Chemistry |
| title | Photometric evaluation of slab gels II: Delimitation and integration of peaks in one‐dimensional electropherograms |
| title_full | Photometric evaluation of slab gels II: Delimitation and integration of peaks in one‐dimensional electropherograms |
| title_fullStr | Photometric evaluation of slab gels II: Delimitation and integration of peaks in one‐dimensional electropherograms |
| title_full_unstemmed | Photometric evaluation of slab gels II: Delimitation and integration of peaks in one‐dimensional electropherograms |
| title_short | Photometric evaluation of slab gels II: Delimitation and integration of peaks in one‐dimensional electropherograms |
| title_sort | photometric evaluation of slab gels ii: delimitation and integration of peaks in one‐dimensional electropherograms |
| title_unstemmed | Photometric evaluation of slab gels II: Delimitation and integration of peaks in one‐dimensional electropherograms |
| topic | Clinical Biochemistry, Biochemistry, Analytical Chemistry |
| url | http://dx.doi.org/10.1002/elps.1150030503 |