author_facet Kronberg, Harald
Zimmer, Hans G.
Neuhoff, Volker
Kronberg, Harald
Zimmer, Hans G.
Neuhoff, Volker
author Kronberg, Harald
Zimmer, Hans G.
Neuhoff, Volker
spellingShingle Kronberg, Harald
Zimmer, Hans G.
Neuhoff, Volker
ELECTROPHORESIS
Photometric evaluation of slab gels I. Data acquisition and image analysis
Clinical Biochemistry
Biochemistry
Analytical Chemistry
author_sort kronberg, harald
spelling Kronberg, Harald Zimmer, Hans G. Neuhoff, Volker 0173-0835 1522-2683 Wiley Clinical Biochemistry Biochemistry Analytical Chemistry http://dx.doi.org/10.1002/elps.1150010106 <jats:title>Abstract</jats:title><jats:p>The photometry of two‐dimensional electrophoretograms is described in some detail. This method has a high sensitivity of 5·10<jats:sup>−4</jats:sup> O.D., because it takes advantage of the noise characteristics of photometric signals. Digital image analysis is used to locate and evaluate the individual spots on the electrophoretogram. The presented procedure of segmentation detects the faintest protein spots visible by eye and separates even confluent spots reliably and automatically. The combination of scanning photometry and image analysis is a sensitive, versatile, and accurate method for the evaluation of slab gels.</jats:p> Photometric evaluation of slab gels I. Data acquisition and image analysis ELECTROPHORESIS
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title Photometric evaluation of slab gels I. Data acquisition and image analysis
title_unstemmed Photometric evaluation of slab gels I. Data acquisition and image analysis
title_full Photometric evaluation of slab gels I. Data acquisition and image analysis
title_fullStr Photometric evaluation of slab gels I. Data acquisition and image analysis
title_full_unstemmed Photometric evaluation of slab gels I. Data acquisition and image analysis
title_short Photometric evaluation of slab gels I. Data acquisition and image analysis
title_sort photometric evaluation of slab gels i. data acquisition and image analysis
topic Clinical Biochemistry
Biochemistry
Analytical Chemistry
url http://dx.doi.org/10.1002/elps.1150010106
publishDate 1980
physical 27-32
description <jats:title>Abstract</jats:title><jats:p>The photometry of two‐dimensional electrophoretograms is described in some detail. This method has a high sensitivity of 5·10<jats:sup>−4</jats:sup> O.D., because it takes advantage of the noise characteristics of photometric signals. Digital image analysis is used to locate and evaluate the individual spots on the electrophoretogram. The presented procedure of segmentation detects the faintest protein spots visible by eye and separates even confluent spots reliably and automatically. The combination of scanning photometry and image analysis is a sensitive, versatile, and accurate method for the evaluation of slab gels.</jats:p>
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author Kronberg, Harald, Zimmer, Hans G., Neuhoff, Volker
author_facet Kronberg, Harald, Zimmer, Hans G., Neuhoff, Volker, Kronberg, Harald, Zimmer, Hans G., Neuhoff, Volker
author_sort kronberg, harald
container_issue 1
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container_title ELECTROPHORESIS
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description <jats:title>Abstract</jats:title><jats:p>The photometry of two‐dimensional electrophoretograms is described in some detail. This method has a high sensitivity of 5·10<jats:sup>−4</jats:sup> O.D., because it takes advantage of the noise characteristics of photometric signals. Digital image analysis is used to locate and evaluate the individual spots on the electrophoretogram. The presented procedure of segmentation detects the faintest protein spots visible by eye and separates even confluent spots reliably and automatically. The combination of scanning photometry and image analysis is a sensitive, versatile, and accurate method for the evaluation of slab gels.</jats:p>
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imprint Wiley, 1980
imprint_str_mv Wiley, 1980
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spelling Kronberg, Harald Zimmer, Hans G. Neuhoff, Volker 0173-0835 1522-2683 Wiley Clinical Biochemistry Biochemistry Analytical Chemistry http://dx.doi.org/10.1002/elps.1150010106 <jats:title>Abstract</jats:title><jats:p>The photometry of two‐dimensional electrophoretograms is described in some detail. This method has a high sensitivity of 5·10<jats:sup>−4</jats:sup> O.D., because it takes advantage of the noise characteristics of photometric signals. Digital image analysis is used to locate and evaluate the individual spots on the electrophoretogram. The presented procedure of segmentation detects the faintest protein spots visible by eye and separates even confluent spots reliably and automatically. The combination of scanning photometry and image analysis is a sensitive, versatile, and accurate method for the evaluation of slab gels.</jats:p> Photometric evaluation of slab gels I. Data acquisition and image analysis ELECTROPHORESIS
spellingShingle Kronberg, Harald, Zimmer, Hans G., Neuhoff, Volker, ELECTROPHORESIS, Photometric evaluation of slab gels I. Data acquisition and image analysis, Clinical Biochemistry, Biochemistry, Analytical Chemistry
title Photometric evaluation of slab gels I. Data acquisition and image analysis
title_full Photometric evaluation of slab gels I. Data acquisition and image analysis
title_fullStr Photometric evaluation of slab gels I. Data acquisition and image analysis
title_full_unstemmed Photometric evaluation of slab gels I. Data acquisition and image analysis
title_short Photometric evaluation of slab gels I. Data acquisition and image analysis
title_sort photometric evaluation of slab gels i. data acquisition and image analysis
title_unstemmed Photometric evaluation of slab gels I. Data acquisition and image analysis
topic Clinical Biochemistry, Biochemistry, Analytical Chemistry
url http://dx.doi.org/10.1002/elps.1150010106