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Photometric evaluation of slab gels I. Data acquisition and image analysis
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Zeitschriftentitel: | ELECTROPHORESIS |
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Personen und Körperschaften: | , , |
In: | ELECTROPHORESIS, 1, 1980, 1, S. 27-32 |
Format: | E-Article |
Sprache: | Englisch |
veröffentlicht: |
Wiley
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Schlagwörter: |
author_facet |
Kronberg, Harald Zimmer, Hans G. Neuhoff, Volker Kronberg, Harald Zimmer, Hans G. Neuhoff, Volker |
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author |
Kronberg, Harald Zimmer, Hans G. Neuhoff, Volker |
spellingShingle |
Kronberg, Harald Zimmer, Hans G. Neuhoff, Volker ELECTROPHORESIS Photometric evaluation of slab gels I. Data acquisition and image analysis Clinical Biochemistry Biochemistry Analytical Chemistry |
author_sort |
kronberg, harald |
spelling |
Kronberg, Harald Zimmer, Hans G. Neuhoff, Volker 0173-0835 1522-2683 Wiley Clinical Biochemistry Biochemistry Analytical Chemistry http://dx.doi.org/10.1002/elps.1150010106 <jats:title>Abstract</jats:title><jats:p>The photometry of two‐dimensional electrophoretograms is described in some detail. This method has a high sensitivity of 5·10<jats:sup>−4</jats:sup> O.D., because it takes advantage of the noise characteristics of photometric signals. Digital image analysis is used to locate and evaluate the individual spots on the electrophoretogram. The presented procedure of segmentation detects the faintest protein spots visible by eye and separates even confluent spots reliably and automatically. The combination of scanning photometry and image analysis is a sensitive, versatile, and accurate method for the evaluation of slab gels.</jats:p> Photometric evaluation of slab gels I. Data acquisition and image analysis ELECTROPHORESIS |
doi_str_mv |
10.1002/elps.1150010106 |
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Online |
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Medizin Biologie Chemie und Pharmazie |
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Wiley, 1980 |
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Wiley, 1980 |
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1980 |
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Wiley |
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ai |
series |
ELECTROPHORESIS |
source_id |
49 |
title |
Photometric evaluation of slab gels I. Data acquisition and image analysis |
title_unstemmed |
Photometric evaluation of slab gels I. Data acquisition and image analysis |
title_full |
Photometric evaluation of slab gels I. Data acquisition and image analysis |
title_fullStr |
Photometric evaluation of slab gels I. Data acquisition and image analysis |
title_full_unstemmed |
Photometric evaluation of slab gels I. Data acquisition and image analysis |
title_short |
Photometric evaluation of slab gels I. Data acquisition and image analysis |
title_sort |
photometric evaluation of slab gels i. data acquisition and image analysis |
topic |
Clinical Biochemistry Biochemistry Analytical Chemistry |
url |
http://dx.doi.org/10.1002/elps.1150010106 |
publishDate |
1980 |
physical |
27-32 |
description |
<jats:title>Abstract</jats:title><jats:p>The photometry of two‐dimensional electrophoretograms is described in some detail. This method has a high sensitivity of 5·10<jats:sup>−4</jats:sup> O.D., because it takes advantage of the noise characteristics of photometric signals. Digital image analysis is used to locate and evaluate the individual spots on the electrophoretogram. The presented procedure of segmentation detects the faintest protein spots visible by eye and separates even confluent spots reliably and automatically. The combination of scanning photometry and image analysis is a sensitive, versatile, and accurate method for the evaluation of slab gels.</jats:p> |
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author | Kronberg, Harald, Zimmer, Hans G., Neuhoff, Volker |
author_facet | Kronberg, Harald, Zimmer, Hans G., Neuhoff, Volker, Kronberg, Harald, Zimmer, Hans G., Neuhoff, Volker |
author_sort | kronberg, harald |
container_issue | 1 |
container_start_page | 27 |
container_title | ELECTROPHORESIS |
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description | <jats:title>Abstract</jats:title><jats:p>The photometry of two‐dimensional electrophoretograms is described in some detail. This method has a high sensitivity of 5·10<jats:sup>−4</jats:sup> O.D., because it takes advantage of the noise characteristics of photometric signals. Digital image analysis is used to locate and evaluate the individual spots on the electrophoretogram. The presented procedure of segmentation detects the faintest protein spots visible by eye and separates even confluent spots reliably and automatically. The combination of scanning photometry and image analysis is a sensitive, versatile, and accurate method for the evaluation of slab gels.</jats:p> |
doi_str_mv | 10.1002/elps.1150010106 |
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finc_class_facet | Medizin, Biologie, Chemie und Pharmazie |
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id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9lbHBzLjExNTAwMTAxMDY |
imprint | Wiley, 1980 |
imprint_str_mv | Wiley, 1980 |
institution | DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161 |
issn | 0173-0835, 1522-2683 |
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physical | 27-32 |
publishDate | 1980 |
publishDateSort | 1980 |
publisher | Wiley |
record_format | ai |
recordtype | ai |
series | ELECTROPHORESIS |
source_id | 49 |
spelling | Kronberg, Harald Zimmer, Hans G. Neuhoff, Volker 0173-0835 1522-2683 Wiley Clinical Biochemistry Biochemistry Analytical Chemistry http://dx.doi.org/10.1002/elps.1150010106 <jats:title>Abstract</jats:title><jats:p>The photometry of two‐dimensional electrophoretograms is described in some detail. This method has a high sensitivity of 5·10<jats:sup>−4</jats:sup> O.D., because it takes advantage of the noise characteristics of photometric signals. Digital image analysis is used to locate and evaluate the individual spots on the electrophoretogram. The presented procedure of segmentation detects the faintest protein spots visible by eye and separates even confluent spots reliably and automatically. The combination of scanning photometry and image analysis is a sensitive, versatile, and accurate method for the evaluation of slab gels.</jats:p> Photometric evaluation of slab gels I. Data acquisition and image analysis ELECTROPHORESIS |
spellingShingle | Kronberg, Harald, Zimmer, Hans G., Neuhoff, Volker, ELECTROPHORESIS, Photometric evaluation of slab gels I. Data acquisition and image analysis, Clinical Biochemistry, Biochemistry, Analytical Chemistry |
title | Photometric evaluation of slab gels I. Data acquisition and image analysis |
title_full | Photometric evaluation of slab gels I. Data acquisition and image analysis |
title_fullStr | Photometric evaluation of slab gels I. Data acquisition and image analysis |
title_full_unstemmed | Photometric evaluation of slab gels I. Data acquisition and image analysis |
title_short | Photometric evaluation of slab gels I. Data acquisition and image analysis |
title_sort | photometric evaluation of slab gels i. data acquisition and image analysis |
title_unstemmed | Photometric evaluation of slab gels I. Data acquisition and image analysis |
topic | Clinical Biochemistry, Biochemistry, Analytical Chemistry |
url | http://dx.doi.org/10.1002/elps.1150010106 |