Eintrag weiter verarbeiten
Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces
Gespeichert in:
| Zeitschriftentitel: | Cytometry Part A |
|---|---|
| Personen und Körperschaften: | , , , , , , |
| In: | Cytometry Part A, 91, 2017, 8, S. 775-784 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
|
| Schlagwörter: |
| author_facet |
Guo, Yuting Stärk, Hans J. Hause, Gerd Schmidt, Matthias Harms, Hauke Wick, Lukas Y. Müller, Susann Guo, Yuting Stärk, Hans J. Hause, Gerd Schmidt, Matthias Harms, Hauke Wick, Lukas Y. Müller, Susann |
|---|---|
| author |
Guo, Yuting Stärk, Hans J. Hause, Gerd Schmidt, Matthias Harms, Hauke Wick, Lukas Y. Müller, Susann |
| spellingShingle |
Guo, Yuting Stärk, Hans J. Hause, Gerd Schmidt, Matthias Harms, Hauke Wick, Lukas Y. Müller, Susann Cytometry Part A Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces Cell Biology Histology Pathology and Forensic Medicine |
| author_sort |
guo, yuting |
| spelling |
Guo, Yuting Stärk, Hans J. Hause, Gerd Schmidt, Matthias Harms, Hauke Wick, Lukas Y. Müller, Susann 1552-4922 1552-4930 Wiley Cell Biology Histology Pathology and Forensic Medicine http://dx.doi.org/10.1002/cyto.a.23055 <jats:title>Abstract</jats:title><jats:p>Tons of anthropogenic silver nanoparticles (AgNPs) are assumed to be released into the environment due to their use in many consumer products. AgNPs are known to be toxic toward microorganisms and thus may harm their specific functions in ecosystems. Here we explore the impact of AgNPs on functioning of single cells in microbial populations at doses typically found in anthropogenic environments. The response of single cells to AgNPs was analyzed by flow cytometry and using the fluorescent dyes propidium iodide and DiBAC<jats:sub>4</jats:sub>(3) as markers for cell membrane disintegration and depolarization, respectively. The effects of 10‐nm and 30‐nm AgNPs on three bacterial species (<jats:italic>Mycobacterium frederiksbergense</jats:italic>, <jats:italic>Pseudomonas putida</jats:italic>, and <jats:italic>Escherichia coli</jats:italic>) showed that the populations split into affected cells and others not showing any malfunction, with varying abundances depending on strains and cell growth states. Further, the dissolution of AgNPs measured with 3 KDa ultrafiltration and inductively coupled plasma‐mass‐spectrometry to distinguish particle‐related effects from toxicity of dissolved Ag revealed that Ag ions were the principal toxicant. AgNP aggregate formation was followed by dynamic light scattering and the aggregates’ attachment to cell surfaces was visualized by transmission electron microscopy and scanning electron microscopy‐energy dispersive X‐ray spectroscopy. An increased AgNP‐affected cell fraction relative to the Ag ion impact was identified. The study shows that individual cells in a population cope differently with AgNP induced stress by evolving heterogeneous phenotypes. The response is linked to cell death and cell energy depletion depending on cell type and cell growth states. The attachment of AgNP aggregates to cell surfaces seems to amplify the heterogeneous response. © 2017 International Society for Advancement of Cytometry</jats:p> Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces Cytometry Part A |
| doi_str_mv |
10.1002/cyto.a.23055 |
| facet_avail |
Online Free |
| finc_class_facet |
Biologie Medizin |
| format |
ElectronicArticle |
| fullrecord |
blob:ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9jeXRvLmEuMjMwNTU |
| id |
ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9jeXRvLmEuMjMwNTU |
| institution |
DE-15 DE-Pl11 DE-Rs1 DE-105 DE-14 DE-Ch1 DE-L229 DE-D275 DE-Bn3 DE-Brt1 DE-D161 DE-Zwi2 DE-Gla1 DE-Zi4 |
| imprint |
Wiley, 2017 |
| imprint_str_mv |
Wiley, 2017 |
| issn |
1552-4930 1552-4922 |
| issn_str_mv |
1552-4930 1552-4922 |
| language |
English |
| mega_collection |
Wiley (CrossRef) |
| match_str |
guo2017heterogenicresponseofprokaryotestowardsilvernanoparticlesandionsisfacilitatedbyphenotypesandattachmentofsilveraggregatestocellsurfaces |
| publishDateSort |
2017 |
| publisher |
Wiley |
| recordtype |
ai |
| record_format |
ai |
| series |
Cytometry Part A |
| source_id |
49 |
| title |
Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces |
| title_unstemmed |
Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces |
| title_full |
Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces |
| title_fullStr |
Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces |
| title_full_unstemmed |
Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces |
| title_short |
Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces |
| title_sort |
heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces |
| topic |
Cell Biology Histology Pathology and Forensic Medicine |
| url |
http://dx.doi.org/10.1002/cyto.a.23055 |
| publishDate |
2017 |
| physical |
775-784 |
| description |
<jats:title>Abstract</jats:title><jats:p>Tons of anthropogenic silver nanoparticles (AgNPs) are assumed to be released into the environment due to their use in many consumer products. AgNPs are known to be toxic toward microorganisms and thus may harm their specific functions in ecosystems. Here we explore the impact of AgNPs on functioning of single cells in microbial populations at doses typically found in anthropogenic environments. The response of single cells to AgNPs was analyzed by flow cytometry and using the fluorescent dyes propidium iodide and DiBAC<jats:sub>4</jats:sub>(3) as markers for cell membrane disintegration and depolarization, respectively. The effects of 10‐nm and 30‐nm AgNPs on three bacterial species (<jats:italic>Mycobacterium frederiksbergense</jats:italic>, <jats:italic>Pseudomonas putida</jats:italic>, and <jats:italic>Escherichia coli</jats:italic>) showed that the populations split into affected cells and others not showing any malfunction, with varying abundances depending on strains and cell growth states. Further, the dissolution of AgNPs measured with 3 KDa ultrafiltration and inductively coupled plasma‐mass‐spectrometry to distinguish particle‐related effects from toxicity of dissolved Ag revealed that Ag ions were the principal toxicant. AgNP aggregate formation was followed by dynamic light scattering and the aggregates’ attachment to cell surfaces was visualized by transmission electron microscopy and scanning electron microscopy‐energy dispersive X‐ray spectroscopy. An increased AgNP‐affected cell fraction relative to the Ag ion impact was identified. The study shows that individual cells in a population cope differently with AgNP induced stress by evolving heterogeneous phenotypes. The response is linked to cell death and cell energy depletion depending on cell type and cell growth states. The attachment of AgNP aggregates to cell surfaces seems to amplify the heterogeneous response. © 2017 International Society for Advancement of Cytometry</jats:p> |
| container_issue |
8 |
| container_start_page |
775 |
| container_title |
Cytometry Part A |
| container_volume |
91 |
| format_de105 |
Article, E-Article |
| format_de14 |
Article, E-Article |
| format_de15 |
Article, E-Article |
| format_de520 |
Article, E-Article |
| format_de540 |
Article, E-Article |
| format_dech1 |
Article, E-Article |
| format_ded117 |
Article, E-Article |
| format_degla1 |
E-Article |
| format_del152 |
Buch |
| format_del189 |
Article, E-Article |
| format_dezi4 |
Article |
| format_dezwi2 |
Article, E-Article |
| format_finc |
Article, E-Article |
| format_nrw |
Article, E-Article |
| _version_ |
1792333585380802560 |
| geogr_code |
not assigned |
| last_indexed |
2024-03-01T14:15:06.678Z |
| geogr_code_person |
not assigned |
| openURL |
url_ver=Z39.88-2004&ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fvufind.svn.sourceforge.net%3Agenerator&rft.title=Heterogenic+response+of+prokaryotes+toward+silver+nanoparticles+and+ions+is+facilitated+by+phenotypes+and+attachment+of+silver+aggregates+to+cell+surfaces&rft.date=2017-08-01&genre=article&issn=1552-4930&volume=91&issue=8&spage=775&epage=784&pages=775-784&jtitle=Cytometry+Part+A&atitle=Heterogenic+response+of+prokaryotes+toward+silver+nanoparticles+and+ions+is+facilitated+by+phenotypes+and+attachment+of+silver+aggregates+to+cell+surfaces&aulast=M%C3%BCller&aufirst=Susann&rft_id=info%3Adoi%2F10.1002%2Fcyto.a.23055&rft.language%5B0%5D=eng |
| SOLR | |
| _version_ | 1792333585380802560 |
| author | Guo, Yuting, Stärk, Hans J., Hause, Gerd, Schmidt, Matthias, Harms, Hauke, Wick, Lukas Y., Müller, Susann |
| author_facet | Guo, Yuting, Stärk, Hans J., Hause, Gerd, Schmidt, Matthias, Harms, Hauke, Wick, Lukas Y., Müller, Susann, Guo, Yuting, Stärk, Hans J., Hause, Gerd, Schmidt, Matthias, Harms, Hauke, Wick, Lukas Y., Müller, Susann |
| author_sort | guo, yuting |
| container_issue | 8 |
| container_start_page | 775 |
| container_title | Cytometry Part A |
| container_volume | 91 |
| description | <jats:title>Abstract</jats:title><jats:p>Tons of anthropogenic silver nanoparticles (AgNPs) are assumed to be released into the environment due to their use in many consumer products. AgNPs are known to be toxic toward microorganisms and thus may harm their specific functions in ecosystems. Here we explore the impact of AgNPs on functioning of single cells in microbial populations at doses typically found in anthropogenic environments. The response of single cells to AgNPs was analyzed by flow cytometry and using the fluorescent dyes propidium iodide and DiBAC<jats:sub>4</jats:sub>(3) as markers for cell membrane disintegration and depolarization, respectively. The effects of 10‐nm and 30‐nm AgNPs on three bacterial species (<jats:italic>Mycobacterium frederiksbergense</jats:italic>, <jats:italic>Pseudomonas putida</jats:italic>, and <jats:italic>Escherichia coli</jats:italic>) showed that the populations split into affected cells and others not showing any malfunction, with varying abundances depending on strains and cell growth states. Further, the dissolution of AgNPs measured with 3 KDa ultrafiltration and inductively coupled plasma‐mass‐spectrometry to distinguish particle‐related effects from toxicity of dissolved Ag revealed that Ag ions were the principal toxicant. AgNP aggregate formation was followed by dynamic light scattering and the aggregates’ attachment to cell surfaces was visualized by transmission electron microscopy and scanning electron microscopy‐energy dispersive X‐ray spectroscopy. An increased AgNP‐affected cell fraction relative to the Ag ion impact was identified. The study shows that individual cells in a population cope differently with AgNP induced stress by evolving heterogeneous phenotypes. The response is linked to cell death and cell energy depletion depending on cell type and cell growth states. The attachment of AgNP aggregates to cell surfaces seems to amplify the heterogeneous response. © 2017 International Society for Advancement of Cytometry</jats:p> |
| doi_str_mv | 10.1002/cyto.a.23055 |
| facet_avail | Online, Free |
| finc_class_facet | Biologie, Medizin |
| format | ElectronicArticle |
| format_de105 | Article, E-Article |
| format_de14 | Article, E-Article |
| format_de15 | Article, E-Article |
| format_de520 | Article, E-Article |
| format_de540 | Article, E-Article |
| format_dech1 | Article, E-Article |
| format_ded117 | Article, E-Article |
| format_degla1 | E-Article |
| format_del152 | Buch |
| format_del189 | Article, E-Article |
| format_dezi4 | Article |
| format_dezwi2 | Article, E-Article |
| format_finc | Article, E-Article |
| format_nrw | Article, E-Article |
| geogr_code | not assigned |
| geogr_code_person | not assigned |
| id | ai-49-aHR0cDovL2R4LmRvaS5vcmcvMTAuMTAwMi9jeXRvLmEuMjMwNTU |
| imprint | Wiley, 2017 |
| imprint_str_mv | Wiley, 2017 |
| institution | DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4 |
| issn | 1552-4930, 1552-4922 |
| issn_str_mv | 1552-4930, 1552-4922 |
| language | English |
| last_indexed | 2024-03-01T14:15:06.678Z |
| match_str | guo2017heterogenicresponseofprokaryotestowardsilvernanoparticlesandionsisfacilitatedbyphenotypesandattachmentofsilveraggregatestocellsurfaces |
| mega_collection | Wiley (CrossRef) |
| physical | 775-784 |
| publishDate | 2017 |
| publishDateSort | 2017 |
| publisher | Wiley |
| record_format | ai |
| recordtype | ai |
| series | Cytometry Part A |
| source_id | 49 |
| spelling | Guo, Yuting Stärk, Hans J. Hause, Gerd Schmidt, Matthias Harms, Hauke Wick, Lukas Y. Müller, Susann 1552-4922 1552-4930 Wiley Cell Biology Histology Pathology and Forensic Medicine http://dx.doi.org/10.1002/cyto.a.23055 <jats:title>Abstract</jats:title><jats:p>Tons of anthropogenic silver nanoparticles (AgNPs) are assumed to be released into the environment due to their use in many consumer products. AgNPs are known to be toxic toward microorganisms and thus may harm their specific functions in ecosystems. Here we explore the impact of AgNPs on functioning of single cells in microbial populations at doses typically found in anthropogenic environments. The response of single cells to AgNPs was analyzed by flow cytometry and using the fluorescent dyes propidium iodide and DiBAC<jats:sub>4</jats:sub>(3) as markers for cell membrane disintegration and depolarization, respectively. The effects of 10‐nm and 30‐nm AgNPs on three bacterial species (<jats:italic>Mycobacterium frederiksbergense</jats:italic>, <jats:italic>Pseudomonas putida</jats:italic>, and <jats:italic>Escherichia coli</jats:italic>) showed that the populations split into affected cells and others not showing any malfunction, with varying abundances depending on strains and cell growth states. Further, the dissolution of AgNPs measured with 3 KDa ultrafiltration and inductively coupled plasma‐mass‐spectrometry to distinguish particle‐related effects from toxicity of dissolved Ag revealed that Ag ions were the principal toxicant. AgNP aggregate formation was followed by dynamic light scattering and the aggregates’ attachment to cell surfaces was visualized by transmission electron microscopy and scanning electron microscopy‐energy dispersive X‐ray spectroscopy. An increased AgNP‐affected cell fraction relative to the Ag ion impact was identified. The study shows that individual cells in a population cope differently with AgNP induced stress by evolving heterogeneous phenotypes. The response is linked to cell death and cell energy depletion depending on cell type and cell growth states. The attachment of AgNP aggregates to cell surfaces seems to amplify the heterogeneous response. © 2017 International Society for Advancement of Cytometry</jats:p> Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces Cytometry Part A |
| spellingShingle | Guo, Yuting, Stärk, Hans J., Hause, Gerd, Schmidt, Matthias, Harms, Hauke, Wick, Lukas Y., Müller, Susann, Cytometry Part A, Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces, Cell Biology, Histology, Pathology and Forensic Medicine |
| title | Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces |
| title_full | Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces |
| title_fullStr | Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces |
| title_full_unstemmed | Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces |
| title_short | Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces |
| title_sort | heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces |
| title_unstemmed | Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces |
| topic | Cell Biology, Histology, Pathology and Forensic Medicine |
| url | http://dx.doi.org/10.1002/cyto.a.23055 |