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Development of polymorphic simple sequence repeat markers in Huperzia serrata (Lycopodiaceae)
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| Zeitschriftentitel: | Applications in Plant Sciences |
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| Personen und Körperschaften: | , , , , , , , , , , |
| In: | Applications in Plant Sciences, 7, 2019, 7 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Wiley
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| Schlagwörter: |
| author_facet |
Guo, Bin Ren, Jing‐yu He, Mei‐na Yao, Kai Wang, Tian‐shu Wang, Li‐qing Liu, Xin He, Wei Fu, Yan‐ping Wang, De‐li Wei, Ya‐hui Guo, Bin Ren, Jing‐yu He, Mei‐na Yao, Kai Wang, Tian‐shu Wang, Li‐qing Liu, Xin He, Wei Fu, Yan‐ping Wang, De‐li Wei, Ya‐hui |
|---|---|
| author |
Guo, Bin Ren, Jing‐yu He, Mei‐na Yao, Kai Wang, Tian‐shu Wang, Li‐qing Liu, Xin He, Wei Fu, Yan‐ping Wang, De‐li Wei, Ya‐hui |
| spellingShingle |
Guo, Bin Ren, Jing‐yu He, Mei‐na Yao, Kai Wang, Tian‐shu Wang, Li‐qing Liu, Xin He, Wei Fu, Yan‐ping Wang, De‐li Wei, Ya‐hui Applications in Plant Sciences Development of polymorphic simple sequence repeat markers in Huperzia serrata (Lycopodiaceae) Plant Science Ecology, Evolution, Behavior and Systematics |
| author_sort |
guo, bin |
| spelling |
Guo, Bin Ren, Jing‐yu He, Mei‐na Yao, Kai Wang, Tian‐shu Wang, Li‐qing Liu, Xin He, Wei Fu, Yan‐ping Wang, De‐li Wei, Ya‐hui 2168-0450 2168-0450 Wiley Plant Science Ecology, Evolution, Behavior and Systematics http://dx.doi.org/10.1002/aps3.11273 <jats:sec><jats:title>Premise</jats:title><jats:p>The natural population size of <jats:italic>Huperzia serrata</jats:italic> (Lycopodiaceae) has dramatically decreased and the species has become endangered due to overexploitation. Here, we developed simple sequence repeat (<jats:styled-content style="fixed-case">SSR</jats:styled-content>) markers for <jats:italic>H. serrata</jats:italic> to survey both its genetic diversity and population structure.</jats:p></jats:sec><jats:sec><jats:title>Methods and Results</jats:title><jats:p>Based on 177 individuals, 120 <jats:styled-content style="fixed-case">SSR</jats:styled-content> primer pairs were developed and optimized from five regions of the <jats:italic>H. serrata</jats:italic> transcriptomic data. Of these primer pairs, 20 were successfully amplified and 10 showed obvious polymorphism. These polymorphic loci were investigated to study the genetic diversity of <jats:italic>H. serrata</jats:italic>. Two to 11 alleles per locus were identified, the level of observed heterozygosity ranged from 0.00 to 1.00, and the level of expected heterozygosity ranged from 0.19 to 0.79. All loci were successfully amplified in <jats:italic>H. crispata</jats:italic>,<jats:italic> H. sutchueniana</jats:italic>, and <jats:italic>H. selago</jats:italic>.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>The 10 polymorphic primer pairs developed here will be valuable for studies of the endangered <jats:italic>H. serrata</jats:italic> and other related species.</jats:p></jats:sec> Development of polymorphic simple sequence repeat markers in <i>Huperzia serrata</i> (Lycopodiaceae) Applications in Plant Sciences |
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10.1002/aps3.11273 |
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Applications in Plant Sciences |
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49 |
| title |
Development of polymorphic simple sequence repeat markers in Huperzia serrata (Lycopodiaceae) |
| title_unstemmed |
Development of polymorphic simple sequence repeat markers in Huperzia serrata (Lycopodiaceae) |
| title_full |
Development of polymorphic simple sequence repeat markers in Huperzia serrata (Lycopodiaceae) |
| title_fullStr |
Development of polymorphic simple sequence repeat markers in Huperzia serrata (Lycopodiaceae) |
| title_full_unstemmed |
Development of polymorphic simple sequence repeat markers in Huperzia serrata (Lycopodiaceae) |
| title_short |
Development of polymorphic simple sequence repeat markers in Huperzia serrata (Lycopodiaceae) |
| title_sort |
development of polymorphic simple sequence repeat markers in <i>huperzia serrata</i> (lycopodiaceae) |
| topic |
Plant Science Ecology, Evolution, Behavior and Systematics |
| url |
http://dx.doi.org/10.1002/aps3.11273 |
| publishDate |
2019 |
| physical |
|
| description |
<jats:sec><jats:title>Premise</jats:title><jats:p>The natural population size of <jats:italic>Huperzia serrata</jats:italic> (Lycopodiaceae) has dramatically decreased and the species has become endangered due to overexploitation. Here, we developed simple sequence repeat (<jats:styled-content style="fixed-case">SSR</jats:styled-content>) markers for <jats:italic>H. serrata</jats:italic> to survey both its genetic diversity and population structure.</jats:p></jats:sec><jats:sec><jats:title>Methods and Results</jats:title><jats:p>Based on 177 individuals, 120 <jats:styled-content style="fixed-case">SSR</jats:styled-content> primer pairs were developed and optimized from five regions of the <jats:italic>H. serrata</jats:italic> transcriptomic data. Of these primer pairs, 20 were successfully amplified and 10 showed obvious polymorphism. These polymorphic loci were investigated to study the genetic diversity of <jats:italic>H. serrata</jats:italic>. Two to 11 alleles per locus were identified, the level of observed heterozygosity ranged from 0.00 to 1.00, and the level of expected heterozygosity ranged from 0.19 to 0.79. All loci were successfully amplified in <jats:italic>H. crispata</jats:italic>,<jats:italic> H. sutchueniana</jats:italic>, and <jats:italic>H. selago</jats:italic>.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>The 10 polymorphic primer pairs developed here will be valuable for studies of the endangered <jats:italic>H. serrata</jats:italic> and other related species.</jats:p></jats:sec> |
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| author | Guo, Bin, Ren, Jing‐yu, He, Mei‐na, Yao, Kai, Wang, Tian‐shu, Wang, Li‐qing, Liu, Xin, He, Wei, Fu, Yan‐ping, Wang, De‐li, Wei, Ya‐hui |
| author_facet | Guo, Bin, Ren, Jing‐yu, He, Mei‐na, Yao, Kai, Wang, Tian‐shu, Wang, Li‐qing, Liu, Xin, He, Wei, Fu, Yan‐ping, Wang, De‐li, Wei, Ya‐hui, Guo, Bin, Ren, Jing‐yu, He, Mei‐na, Yao, Kai, Wang, Tian‐shu, Wang, Li‐qing, Liu, Xin, He, Wei, Fu, Yan‐ping, Wang, De‐li, Wei, Ya‐hui |
| author_sort | guo, bin |
| container_issue | 7 |
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| description | <jats:sec><jats:title>Premise</jats:title><jats:p>The natural population size of <jats:italic>Huperzia serrata</jats:italic> (Lycopodiaceae) has dramatically decreased and the species has become endangered due to overexploitation. Here, we developed simple sequence repeat (<jats:styled-content style="fixed-case">SSR</jats:styled-content>) markers for <jats:italic>H. serrata</jats:italic> to survey both its genetic diversity and population structure.</jats:p></jats:sec><jats:sec><jats:title>Methods and Results</jats:title><jats:p>Based on 177 individuals, 120 <jats:styled-content style="fixed-case">SSR</jats:styled-content> primer pairs were developed and optimized from five regions of the <jats:italic>H. serrata</jats:italic> transcriptomic data. Of these primer pairs, 20 were successfully amplified and 10 showed obvious polymorphism. These polymorphic loci were investigated to study the genetic diversity of <jats:italic>H. serrata</jats:italic>. Two to 11 alleles per locus were identified, the level of observed heterozygosity ranged from 0.00 to 1.00, and the level of expected heterozygosity ranged from 0.19 to 0.79. All loci were successfully amplified in <jats:italic>H. crispata</jats:italic>,<jats:italic> H. sutchueniana</jats:italic>, and <jats:italic>H. selago</jats:italic>.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>The 10 polymorphic primer pairs developed here will be valuable for studies of the endangered <jats:italic>H. serrata</jats:italic> and other related species.</jats:p></jats:sec> |
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| institution | DE-D275, DE-Bn3, DE-Brt1, DE-D161, DE-Zwi2, DE-Gla1, DE-Zi4, DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229 |
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| spelling | Guo, Bin Ren, Jing‐yu He, Mei‐na Yao, Kai Wang, Tian‐shu Wang, Li‐qing Liu, Xin He, Wei Fu, Yan‐ping Wang, De‐li Wei, Ya‐hui 2168-0450 2168-0450 Wiley Plant Science Ecology, Evolution, Behavior and Systematics http://dx.doi.org/10.1002/aps3.11273 <jats:sec><jats:title>Premise</jats:title><jats:p>The natural population size of <jats:italic>Huperzia serrata</jats:italic> (Lycopodiaceae) has dramatically decreased and the species has become endangered due to overexploitation. Here, we developed simple sequence repeat (<jats:styled-content style="fixed-case">SSR</jats:styled-content>) markers for <jats:italic>H. serrata</jats:italic> to survey both its genetic diversity and population structure.</jats:p></jats:sec><jats:sec><jats:title>Methods and Results</jats:title><jats:p>Based on 177 individuals, 120 <jats:styled-content style="fixed-case">SSR</jats:styled-content> primer pairs were developed and optimized from five regions of the <jats:italic>H. serrata</jats:italic> transcriptomic data. Of these primer pairs, 20 were successfully amplified and 10 showed obvious polymorphism. These polymorphic loci were investigated to study the genetic diversity of <jats:italic>H. serrata</jats:italic>. Two to 11 alleles per locus were identified, the level of observed heterozygosity ranged from 0.00 to 1.00, and the level of expected heterozygosity ranged from 0.19 to 0.79. All loci were successfully amplified in <jats:italic>H. crispata</jats:italic>,<jats:italic> H. sutchueniana</jats:italic>, and <jats:italic>H. selago</jats:italic>.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>The 10 polymorphic primer pairs developed here will be valuable for studies of the endangered <jats:italic>H. serrata</jats:italic> and other related species.</jats:p></jats:sec> Development of polymorphic simple sequence repeat markers in <i>Huperzia serrata</i> (Lycopodiaceae) Applications in Plant Sciences |
| spellingShingle | Guo, Bin, Ren, Jing‐yu, He, Mei‐na, Yao, Kai, Wang, Tian‐shu, Wang, Li‐qing, Liu, Xin, He, Wei, Fu, Yan‐ping, Wang, De‐li, Wei, Ya‐hui, Applications in Plant Sciences, Development of polymorphic simple sequence repeat markers in Huperzia serrata (Lycopodiaceae), Plant Science, Ecology, Evolution, Behavior and Systematics |
| title | Development of polymorphic simple sequence repeat markers in Huperzia serrata (Lycopodiaceae) |
| title_full | Development of polymorphic simple sequence repeat markers in Huperzia serrata (Lycopodiaceae) |
| title_fullStr | Development of polymorphic simple sequence repeat markers in Huperzia serrata (Lycopodiaceae) |
| title_full_unstemmed | Development of polymorphic simple sequence repeat markers in Huperzia serrata (Lycopodiaceae) |
| title_short | Development of polymorphic simple sequence repeat markers in Huperzia serrata (Lycopodiaceae) |
| title_sort | development of polymorphic simple sequence repeat markers in <i>huperzia serrata</i> (lycopodiaceae) |
| title_unstemmed | Development of polymorphic simple sequence repeat markers in Huperzia serrata (Lycopodiaceae) |
| topic | Plant Science, Ecology, Evolution, Behavior and Systematics |
| url | http://dx.doi.org/10.1002/aps3.11273 |