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Osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells
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| Zeitschriftentitel: | Bioscience Reports |
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| Personen und Körperschaften: | , , , , , , , , , , , , |
| In: | Bioscience Reports, 39, 2019, 4 |
| Format: | E-Article |
| Sprache: | Englisch |
| veröffentlicht: |
Portland Press Ltd.
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| Schlagwörter: |
| author_facet |
Liu, Ziming Zhang, Zhiwen Zhang, Ali Zhang, Fan Du, Wennan Zhang, Yongqiang Zhang, Rongfeng Xu, Jiagang Wu, Xiangkun Zhang, Chengmin Li, Ning Tan, Qi Huang, Wei Liu, Ziming Zhang, Zhiwen Zhang, Ali Zhang, Fan Du, Wennan Zhang, Yongqiang Zhang, Rongfeng Xu, Jiagang Wu, Xiangkun Zhang, Chengmin Li, Ning Tan, Qi Huang, Wei |
|---|---|
| author |
Liu, Ziming Zhang, Zhiwen Zhang, Ali Zhang, Fan Du, Wennan Zhang, Yongqiang Zhang, Rongfeng Xu, Jiagang Wu, Xiangkun Zhang, Chengmin Li, Ning Tan, Qi Huang, Wei |
| spellingShingle |
Liu, Ziming Zhang, Zhiwen Zhang, Ali Zhang, Fan Du, Wennan Zhang, Yongqiang Zhang, Rongfeng Xu, Jiagang Wu, Xiangkun Zhang, Chengmin Li, Ning Tan, Qi Huang, Wei Bioscience Reports Osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells Cell Biology Molecular Biology Biochemistry Biophysics |
| author_sort |
liu, ziming |
| spelling |
Liu, Ziming Zhang, Zhiwen Zhang, Ali Zhang, Fan Du, Wennan Zhang, Yongqiang Zhang, Rongfeng Xu, Jiagang Wu, Xiangkun Zhang, Chengmin Li, Ning Tan, Qi Huang, Wei 0144-8463 1573-4935 Portland Press Ltd. Cell Biology Molecular Biology Biochemistry Biophysics http://dx.doi.org/10.1042/bsr20190170 <jats:title>Abstract</jats:title> <jats:p>Increasing evidence has indicated a close relationship between diabetes mellitus (DM) and disc degeneration. As a potential therapeutic growth factor, osteogenic protein-1 (OP-1) has lots of protective effects on the healthy disc cell’s biology. The present study was aimed to investigate the effects of OP-1 on degenerative changes of nucleus pulposus (NP) cells in a high glucose culture. Rat NP cells were cultured in the baseline medium or the high glucose (0.2 M) culture medium. OP-1 was added into the high glucose culture medium to investigate whether its has some protective effects against degenerative changes of NP cells in the high glucose culture. NP cell apoptosis ratio, caspase-3/9 activity, expression of apoptosis-related molecules (Bcl-2, Bax, and caspase-3), matrix macromolecules (aggrecan and collagen II), and matrix remodeling enzymes (MMP-3, MMP-13, and ADAMTS-4), and immuno-staining of NP matrix proteins (aggrecan and collagen II) were evaluated. Compared with the baseline culture, high glucose culture significantly increased NP cell apoptosis ratio, caspase-3/9 activity, up-regulated expression of Bax, caspase-3, MMP-3, MMP-13 and ADAMTS-4, down-regulated expression of Bcl-2, aggrecan and collagen II, and decreased staining intensity of aggrecan and collagen II. However, the results of these parameters were partly reversed by the addition of OP-1 in the high glucose culture. OP-1 can alleviate high glucose microenvironment-induced degenerative changes of NP cells. The present study provides that OP-1 may be promising in retarding disc degeneration in DM patients.</jats:p> Osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells Bioscience Reports |
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| title |
Osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells |
| title_unstemmed |
Osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells |
| title_full |
Osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells |
| title_fullStr |
Osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells |
| title_full_unstemmed |
Osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells |
| title_short |
Osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells |
| title_sort |
osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells |
| topic |
Cell Biology Molecular Biology Biochemistry Biophysics |
| url |
http://dx.doi.org/10.1042/bsr20190170 |
| publishDate |
2019 |
| physical |
|
| description |
<jats:title>Abstract</jats:title>
<jats:p>Increasing evidence has indicated a close relationship between diabetes mellitus (DM) and disc degeneration. As a potential therapeutic growth factor, osteogenic protein-1 (OP-1) has lots of protective effects on the healthy disc cell’s biology. The present study was aimed to investigate the effects of OP-1 on degenerative changes of nucleus pulposus (NP) cells in a high glucose culture. Rat NP cells were cultured in the baseline medium or the high glucose (0.2 M) culture medium. OP-1 was added into the high glucose culture medium to investigate whether its has some protective effects against degenerative changes of NP cells in the high glucose culture. NP cell apoptosis ratio, caspase-3/9 activity, expression of apoptosis-related molecules (Bcl-2, Bax, and caspase-3), matrix macromolecules (aggrecan and collagen II), and matrix remodeling enzymes (MMP-3, MMP-13, and ADAMTS-4), and immuno-staining of NP matrix proteins (aggrecan and collagen II) were evaluated. Compared with the baseline culture, high glucose culture significantly increased NP cell apoptosis ratio, caspase-3/9 activity, up-regulated expression of Bax, caspase-3, MMP-3, MMP-13 and ADAMTS-4, down-regulated expression of Bcl-2, aggrecan and collagen II, and decreased staining intensity of aggrecan and collagen II. However, the results of these parameters were partly reversed by the addition of OP-1 in the high glucose culture. OP-1 can alleviate high glucose microenvironment-induced degenerative changes of NP cells. The present study provides that OP-1 may be promising in retarding disc degeneration in DM patients.</jats:p> |
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| author | Liu, Ziming, Zhang, Zhiwen, Zhang, Ali, Zhang, Fan, Du, Wennan, Zhang, Yongqiang, Zhang, Rongfeng, Xu, Jiagang, Wu, Xiangkun, Zhang, Chengmin, Li, Ning, Tan, Qi, Huang, Wei |
| author_facet | Liu, Ziming, Zhang, Zhiwen, Zhang, Ali, Zhang, Fan, Du, Wennan, Zhang, Yongqiang, Zhang, Rongfeng, Xu, Jiagang, Wu, Xiangkun, Zhang, Chengmin, Li, Ning, Tan, Qi, Huang, Wei, Liu, Ziming, Zhang, Zhiwen, Zhang, Ali, Zhang, Fan, Du, Wennan, Zhang, Yongqiang, Zhang, Rongfeng, Xu, Jiagang, Wu, Xiangkun, Zhang, Chengmin, Li, Ning, Tan, Qi, Huang, Wei |
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| description | <jats:title>Abstract</jats:title> <jats:p>Increasing evidence has indicated a close relationship between diabetes mellitus (DM) and disc degeneration. As a potential therapeutic growth factor, osteogenic protein-1 (OP-1) has lots of protective effects on the healthy disc cell’s biology. The present study was aimed to investigate the effects of OP-1 on degenerative changes of nucleus pulposus (NP) cells in a high glucose culture. Rat NP cells were cultured in the baseline medium or the high glucose (0.2 M) culture medium. OP-1 was added into the high glucose culture medium to investigate whether its has some protective effects against degenerative changes of NP cells in the high glucose culture. NP cell apoptosis ratio, caspase-3/9 activity, expression of apoptosis-related molecules (Bcl-2, Bax, and caspase-3), matrix macromolecules (aggrecan and collagen II), and matrix remodeling enzymes (MMP-3, MMP-13, and ADAMTS-4), and immuno-staining of NP matrix proteins (aggrecan and collagen II) were evaluated. Compared with the baseline culture, high glucose culture significantly increased NP cell apoptosis ratio, caspase-3/9 activity, up-regulated expression of Bax, caspase-3, MMP-3, MMP-13 and ADAMTS-4, down-regulated expression of Bcl-2, aggrecan and collagen II, and decreased staining intensity of aggrecan and collagen II. However, the results of these parameters were partly reversed by the addition of OP-1 in the high glucose culture. OP-1 can alleviate high glucose microenvironment-induced degenerative changes of NP cells. The present study provides that OP-1 may be promising in retarding disc degeneration in DM patients.</jats:p> |
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| institution | DE-15, DE-Pl11, DE-Rs1, DE-105, DE-14, DE-Ch1, DE-L229, DE-D275, DE-Bn3, DE-Brt1, DE-Zwi2, DE-D161, DE-Gla1, DE-Zi4 |
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| spelling | Liu, Ziming Zhang, Zhiwen Zhang, Ali Zhang, Fan Du, Wennan Zhang, Yongqiang Zhang, Rongfeng Xu, Jiagang Wu, Xiangkun Zhang, Chengmin Li, Ning Tan, Qi Huang, Wei 0144-8463 1573-4935 Portland Press Ltd. Cell Biology Molecular Biology Biochemistry Biophysics http://dx.doi.org/10.1042/bsr20190170 <jats:title>Abstract</jats:title> <jats:p>Increasing evidence has indicated a close relationship between diabetes mellitus (DM) and disc degeneration. As a potential therapeutic growth factor, osteogenic protein-1 (OP-1) has lots of protective effects on the healthy disc cell’s biology. The present study was aimed to investigate the effects of OP-1 on degenerative changes of nucleus pulposus (NP) cells in a high glucose culture. Rat NP cells were cultured in the baseline medium or the high glucose (0.2 M) culture medium. OP-1 was added into the high glucose culture medium to investigate whether its has some protective effects against degenerative changes of NP cells in the high glucose culture. NP cell apoptosis ratio, caspase-3/9 activity, expression of apoptosis-related molecules (Bcl-2, Bax, and caspase-3), matrix macromolecules (aggrecan and collagen II), and matrix remodeling enzymes (MMP-3, MMP-13, and ADAMTS-4), and immuno-staining of NP matrix proteins (aggrecan and collagen II) were evaluated. Compared with the baseline culture, high glucose culture significantly increased NP cell apoptosis ratio, caspase-3/9 activity, up-regulated expression of Bax, caspase-3, MMP-3, MMP-13 and ADAMTS-4, down-regulated expression of Bcl-2, aggrecan and collagen II, and decreased staining intensity of aggrecan and collagen II. However, the results of these parameters were partly reversed by the addition of OP-1 in the high glucose culture. OP-1 can alleviate high glucose microenvironment-induced degenerative changes of NP cells. The present study provides that OP-1 may be promising in retarding disc degeneration in DM patients.</jats:p> Osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells Bioscience Reports |
| spellingShingle | Liu, Ziming, Zhang, Zhiwen, Zhang, Ali, Zhang, Fan, Du, Wennan, Zhang, Yongqiang, Zhang, Rongfeng, Xu, Jiagang, Wu, Xiangkun, Zhang, Chengmin, Li, Ning, Tan, Qi, Huang, Wei, Bioscience Reports, Osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells, Cell Biology, Molecular Biology, Biochemistry, Biophysics |
| title | Osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells |
| title_full | Osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells |
| title_fullStr | Osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells |
| title_full_unstemmed | Osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells |
| title_short | Osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells |
| title_sort | osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells |
| title_unstemmed | Osteogenic protein-1 alleviates high glucose microenvironment-caused degenerative changes in nucleus pulposus cells |
| topic | Cell Biology, Molecular Biology, Biochemistry, Biophysics |
| url | http://dx.doi.org/10.1042/bsr20190170 |